AB314576

Anti-MMP16 antibody [EPR26961-54] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MMP16 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

C8orf57, MMPX2, MMP16, Matrix metalloproteinase-16, MMP-16, MMP-X2, Membrane-type matrix metalloproteinase 3, Membrane-type-3 matrix metalloproteinase, MT-MMP 3, MTMMP3, MT3-MMP, MT3MMP

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Sandwich ELISA - Anti-MMP16 antibody [EPR26961-54] - BSA and Azide free (Capture) (AB314576)

sELISA

Supplier Data

Sandwich ELISA - Anti-MMP16 antibody [EPR26961-54] - BSA and Azide free (Capture) (AB314576)

Sandwich ELISA of ab314575 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26961-54

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314576 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MMP16. The recommended pair for sandwich ELISA is:

Capture: ab314576, Human MMP16 Capture Antibody (unconjugated)

Detector: ab314577, Human MMP16 Detector Antibody (unconjugated)

The reference range value is 0.16-20 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP16 also known as MT3-MMP (membrane-type 3 matrix metalloproteinase) is an enzyme part of the matrix metalloproteinase family. It plays a mechanical role in the degradation of extracellular matrix components. MMP16 has a molecular mass of 66 kDa and has proteolytic activity that contributes to tissue remodeling processes. This protein is a membrane-bound enzyme expressed in several tissues including the brain lungs and placenta. In the central nervous system it aids in maintaining extracellular environment by breaking down proteins.

Biological function summary

MMP16 is essential in facilitating cellular activities such as migration invasion and angiogenesis. This protein operates as part of a complex that activates other MMPs including pro-MMP2 boosting their enzymatic activity. MMP16 participates in cellular remodeling by regulating the interactions between cells and their matrix which influences cell signaling and tissue morphogenesis.

Pathways

MMP16 integrates into pathways relevant to extracellular matrix remodeling and cell signaling. It is actively involved in the Wnt and MAPK signaling pathways which influence various biological processes including development and cell proliferation. MMP16 also shows interactions with other MMP family members like MMP2 and MMP14 which collectively modulate the local microenvironment and cellular dynamics.

MMP16 is associated with cancer progression and neurodegenerative diseases. In cancer its upregulation correlates with tumor invasion and metastasis owing to its role in degradation of the basement membrane. MMP16 also contributes to neurodegenerative conditions like Alzheimer's Disease by affecting synaptic functions and neuron survival. It interacts with proteins like APP involved in Alzheimer's linking MMP16 to amyloid beta peptide processing and aggregation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

See full target information MMP16

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP16 antibody [EPR26961-54] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-MMP16 antibody [EPR26961-54] - BSA and Azide free (Capture) (AB314576)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise