Anti-MMP16 antibody [EPR29634-23]

• WB

Supplier Data

Western blot - Anti-MMP16 antibody [EPR29634-23] (AB325318)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, liver.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lanes 1-3 : 180 seconds, lanes 4-5 : 37 seconds

All lanes:

Western blot - Anti-MMP16 antibody [EPR29634-23] (ab325318) at 1/1000 dilution

Lane 1:

Human liver cancer tissue lysate at 50 µg

Lane 2:

Human spleen tissue lysate at 50 µg

Lane 3:

Human liver tissue lysate at 50 µg

Lane 4:

Mouse placenta tissue lysate at 50 µg

Lane 5:

Mouse spleen tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 69 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-MMP16 antibody [EPR29634-23] (AB325318)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : A431, DLD-1.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-MMP16 antibody [EPR29634-23] (ab325318) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 69 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MMP16 antibody [EPR29634-23] (AB325318)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human MMP14 (#P50281) or MMP24 (#Q9Y5R2) by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000dilution.

All lanes:

Western blot - Anti-MMP16 antibody [EPR29634-23] (ab325318) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human MMP16 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MMP14 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MMP24 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 69 kDa,20-69 kDa,124 kDa

false

Exposure time: 15s