AB2462

Anti-MMP2 antibody [4D3]

(3 Reviews)

(36 Publications)

Mouse Monoclonal MMP2 antibody. Suitable for WB and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human MMP2 aa 550-600.

View Alternative Names

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

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Supplier Data

Western blot - Anti-MMP2 antibody [4D3] (AB2462)

400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.

All lanes:

Western blot - Anti-MMP2 antibody [4D3] (ab2462)

Predicted band size: 73 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MMP2 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

P08253

Specificity

Binds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.

Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

See full target information MMP2

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

International journal of ophthalmology 15:914-923 PubMed35814898

2022

Evaluation of nintedanib as a new postoperative antiscarring agent in experimental extraocular muscle surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Gozde Bicaklioglu,Dilara Pirhan,Yusufhan Yazir,Gokhan Duruksu,Selenay Furat Rencber,Nursen Yuksel

Cells, tissues, organs 212:185-193 PubMed34781297

2021

Lentivirus-shRNA Mediated Prolyl Hydroxylase 2 Knockdown Increases HIF-1α and Inhibits Nucleus Pulposus Cells Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Fangfang Shi,Chen Yang,Guangfeng Mao,Chunguang Zhou,Limin Liu,Xi Yang,Yueming Song

CNS neuroscience & therapeutics 28:64-76 PubMed34729926

2021

Pharmacological inhibition of epidermal growth factor receptor attenuates intracranial aneurysm formation by modulating the phenotype of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Luo,Haishuang Tang,Zhaolong Zhang,Rui Zhao,Chuanchuan Wang,Wenguang Hou,Qinghai Huang,Jianmin Liu

Experimental and therapeutic medicine 22:1301 PubMed34630656

2021

Honokiol combined with curcumin sensitizes multidrug-resistant human lung adenocarcinoma A549/DDP cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Qi,Xiaojin Chen,Liqun Bian,Han Zhang,Jian Ma

Journal of immunology research 2021:5569354 PubMed33869638

2021

Ethanol Extracts of Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Zheming Li,Jie Wang,Xuelu Jiang,Mengting Xia,Jianfen Wang,Shenyi Lu,Shouye Li,Hanmei Wang

Archives of gynecology and obstetrics 303:1075-1087 PubMed33128584

2020

Circular RNA circ_0000043 promotes endometrial carcinoma progression by regulating miR-1271-5p/CTNND1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dexian Wei,Meirong Tian,Weibo Fan,Xiaojing Zhong,Shuhong Wang,Yahang Chen,Shihong Zhang

OncoTargets and therapy 13:10465-10473 PubMed33116626

2020

Genetic and Clinical Characterization of HOXB2 in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Pan,Wei Liu,Yi Chai,Junhua Wang,Yuqi Zhang

Molecular therapy. Nucleic acids 22:114-123 PubMed32916597

2020

ROS/NF-κB Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haina Xie,Dongyu Xie,Jingxian Zhang,Wenye Jin,Yue Li,Jun Yao,Zhiqiang Pan,Donghao Xie

Ophthalmic research 64:99-107 PubMed32564013

2020

A Comparison of Subconjunctival Wound Healing between Different Methods of Dissecting Subconjunctival Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Ruowen Gong,Chengguo Zuo,Keling Wu,Simin Zhang,Xi Qin,Yangyang Li,Xinbo Gao,Danping Huang,Mingkai Lin

Journal of Cancer 11:1584-1595 PubMed32047564

2020

Effects of Galectin-1 on Biological Behavior in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mandika Chetry,Yizuo Song,Chunyu Pan,Ruyi Li,Jianan Zhang,Xueqiong Zhu

View all publications

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