Anti-MMP2 antibody [6E3F8]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (AB86607)

Immunohistochemistry of human pancreas carcinoma staining MMP2 with ab86607 at 1μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (AB86607)

IHC image of ab86607 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86607, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (AB86607)

Immunohistochemical (parraffin-embedded sections) staining for MMP-2 in atheroslerotic lesions of aortic root in from right to left Ad-MIFi, Ad-EGFP, NS control and non-DM mouse control groups using ab86607 at a dilution of 1/200.

Image from Sun H et al., J Cell Mol Med. 2015;19(4):836-49. Fig 6.; doi: 10.1111/jcmm.12521. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• WB

Lab

Western blot - Anti-MMP2 antibody [6E3F8] (AB86607)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab86607 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

A375 (Human melanoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/50000 dilution

Predicted band size: 73 kDa

Observed band size: 100 kDa,70 kDa,75 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Anti-MMP2 antibody [6E3F8] (AB86607)

All lanes:

Western blot - Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Spleen (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 73 kDa

Observed band size: 17 kDa,75 kDa

true

Exposure time: 20min