Rabbit Polyclonal MMP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
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- Molecules (Basel, Switzerland) 28:2023Effects of Radiation-Induced Skin Injury on Hyaluronan Degradation and Its Underlying Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahui Dong,Boyuan Ren,Yunfei Tian,Guanqun Peng,Huiting Zhai,Zhiyun Meng,Ruolan Gu,Hui Gan,Zhuona Wu,Yunbo Sun,Guifang Dou,Shuchen LiuPubMed 37959868
- Journal of gynecologic oncology 34:e112022Circ_0075960 targets the miR-202-5p/CTNND1 axis to promote the growth and migration of endometrial carcinoma cells via regulating Wnt/β-catenin signaling activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Nian,Xiaorong Li,Jingwen Ma,Ting Gao,Dan LiuPubMed 36424704
- Reproductive biology and endocrinology : RB&E 20:1382022Circ-CSNK1G1 promotes cell proliferation, migration, invasion and glycolysis metabolism during triple-negative breast cancer progression by modulating the miR-28-5p/LDHA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochen Zan,Wenfang Li,Geng Wang,Jie Yuan,Yongbiao Ai,Jun Huang,Zhi LiPubMed 36109751
- Translational vision science & technology 11:302022HL3501, a Novel Selective A3 Adenosine Receptor Antagonist, Lowers Intraocular Pressure (IOP) in Animal Glaucoma Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhee Kim,Jaewook Yang,Jee Young Kim,Jang Mi Lee,Woo Chan Son,Byoung-Gon MoonPubMed 35191964
- Oncology reports 47:2022Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaihua Yan,Yu Zhou,Yang Yang,Chongpu Zeng,Peidong Li,Hongpeng Tian,Xuegui Tang,Guangjun ZhangPubMed 35029289
- Oncology letters 22:7272021LncRNA GAS6 antisense RNA 1 facilitates the tumorigenesis of clear cell renal cell carcinoma by regulating the AMP-activated protein kinase/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun Guo,Hongjun Li,Mei Zhang,Rong LiPubMed 34429767
- International journal of molecular sciences 22:2021Electrophysiological and Structural Remodeling of the Atria in a Mouse Model of Troponin-I Mutation Linked Hypertrophic Cardiomyopathy: Implications for Atrial Fibrillation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Wen Lim,Melissa Neo,Shivshankar Thanigaimani,Pawel Kuklik,Anand N Ganesan,Dennis H Lau,Tatiana Tsoutsman,Jonathan M Kalman,Christopher Semsarian,David A Saint,Prashanthan SandersPubMed 34203369
- OncoTargets and therapy 13:12141-121492020Hepsin Promotes Epithelial-Mesenchymal Transition and Cell Invasion Through the miR-222/PPP2R2A/AKT Axis in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiqian Li et. al.PubMed 33268993
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 15 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.Isoform 2Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Alternative names
CLG4A, CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2
Recommended products
Rabbit Polyclonal MMP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
- Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
- Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
- Associated diseases and disorders
Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab235167)
Immunohistochemical analysis of paraffin-embedded human metastic breast carcinoma tissue in lymph node tissue labeling MMP2 with ab235167 at 1/100 dilution (30 minutes at RT), followed by a ready to use Goat anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MMP2 with ab235167 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 μg/ml (1 hour at RT) (green).
Panel A: DAPI.
Panel B: ab235167 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only control.
Western blot - Anti-MMP2 antibody (ab235167)
Blocking/Dilution buffer: 5% BLOTTO.
All lanes: Western blot - Anti-MMP2 antibody (ab235167) at 1/1000 dilution
Lane 1: MMP2 HEK-293 (human epithelial cell line from embryonic kidney) over-expressing lysate
Lane 2: HEK-293 (empty vector) lysate
SecondaryAll lanes: Pre-adsorbed goat anti-rabbit IgG (H&L) conjugated to HRP at 1/70000 dilution
Predicted band size: 73 kDa
Observed band size: 75 kDa
Exposure time: 3s
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab235167)
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized U87MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling MMP2 with ab235167 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 μg/ml (1 hour at RT) (green).
Panel A: DAPI.
Panel B: ab235167 + secondary antibody.
Panel C: Merge of panel A and B.
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