AB235167

Anti-MMP2 antibody

Name: Anti-MMP2 antibody (ab235167) | Abcam
Brand: Abcam Limited
SKU: AB235167

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(14 Publications)

Rabbit Polyclonal MMP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 14 publications.

View Alternative Names

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB235167)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB235167)

Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MMP2 with ab235167 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 μg/ml (1 hour at RT) (green).
Panel A : DAPI.
Panel B : ab235167 + secondary antibody.
Panel C : Merge of panel A and B.
Panel D : Secondary only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB235167)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB235167)

Immunohistochemical analysis of paraffin-embedded human metastic breast carcinoma tissue in lymph node tissue labeling MMP2 with ab235167 at 1/100 dilution (30 minutes at RT), followed by a ready to use Goat anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Counterstained with hematoxylin.

Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB235167)

Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized U87MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling MMP2 with ab235167 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 μg/ml (1 hour at RT) (green).
Panel A : DAPI.
Panel B : ab235167 + secondary antibody.
Panel C : Merge of panel A and B.

Supplier Data

Western blot - Anti-MMP2 antibody (AB235167)

Blocking/Dilution buffer : 5% BLOTTO.

All lanes:

Western blot - Anti-MMP2 antibody (ab235167) at 1/1000 dilution

Lane 1:

MMP2 HEK-293 (human epithelial cell line from embryonic kidney) over-expressing lysate

Lane 2:

HEK-293 (empty vector) lysate

Secondary

All lanes:

Pre-adsorbed goat anti-rabbit IgG (H&L) conjugated to HRP at 1/70000 dilution

Predicted band size: 73 kDa

Observed band size: 75 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.

Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

See full target information MMP2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:328 PubMed41044542

2025

Tetrahydropalmatine has analgesic role in mouse model of bone cancer pain by inactivating the TNF-α/uPA/PAR2/TRPV1 pathway in dorsal root ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Guangda Zheng,Linghan Meng,Lu Shang,Juanxia Ren,Dongtao Li,Yanju Bao

Physiological reports 13:e70580 PubMed40999314

2025

High-intensity interval training attenuates cardiac injury by targeting ferroptosis and endoplasmic reticulum-stress in male rats with heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Sabouri,Abbasali Gaeini,Soheila Adeli,Alireza Ghardashi Afousi

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20:35 PubMed40214929

2025

Yi-Nao-Jie-Yu Prescription Relieves Post-Stroke Depression by Mitigating Ferroptosis in Hippocampal Neurons Via Activating the Nrf2/GPX4/SLC7A11 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Qisheng Tang,Jin Yao,Hongwei Liu,Changmin Xu,Zechun Guo,Shuqing Liu,Ruizhen Zhao

Open life sciences 19:20220990 PubMed39759103

2025

MFAP5 inhibits the malignant progression of endometrial cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Liang,Zijuan Qi,Chun Du

Molecules (Basel, Switzerland) 28: PubMed37959868

2023

Effects of Radiation-Induced Skin Injury on Hyaluronan Degradation and Its Underlying Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Dong,Boyuan Ren,Yunfei Tian,Guanqun Peng,Huiting Zhai,Zhiyun Meng,Ruolan Gu,Hui Gan,Zhuona Wu,Yunbo Sun,Guifang Dou,Shuchen Liu

Journal of gynecologic oncology 34:e11 PubMed36424704

2022

Circ_0075960 targets the miR-202-5p/CTNND1 axis to promote the growth and migration of endometrial carcinoma cells via regulating Wnt/β-catenin signaling activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Nian,Xiaorong Li,Jingwen Ma,Ting Gao,Dan Liu

Reproductive biology and endocrinology : RB&E 20:138 PubMed36109751

2022

Circ-CSNK1G1 promotes cell proliferation, migration, invasion and glycolysis metabolism during triple-negative breast cancer progression by modulating the miR-28-5p/LDHA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Zan,Wenfang Li,Geng Wang,Jie Yuan,Yongbiao Ai,Jun Huang,Zhi Li

Translational vision science & technology 11:30 PubMed35191964

2022

HL3501, a Novel Selective A3 Adenosine Receptor Antagonist, Lowers Intraocular Pressure (IOP) in Animal Glaucoma Models.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhee Kim,Jaewook Yang,Jee Young Kim,Jang Mi Lee,Woo Chan Son,Byoung-Gon Moon

Oncology reports 47: PubMed35029289

2022

Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2.

Applications

Unspecified application

Species

Unspecified reactive species

Zaihua Yan,Yu Zhou,Yang Yang,Chongpu Zeng,Peidong Li,Hongpeng Tian,Xuegui Tang,Guangjun Zhang

Oncology letters 22:727 PubMed34429767

2021

LncRNA GAS6 antisense RNA 1 facilitates the tumorigenesis of clear cell renal cell carcinoma by regulating the AMP-activated protein kinase/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Guo,Hongjun Li,Mei Zhang,Rong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB235167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB235167)

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB235167)

Western blot - Anti-MMP2 antibody (AB235167)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise