Anti-MMP2 antibody

• WB

Supplier Data

Western blot - Anti-MMP2 antibody (AB97779)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution

Lane 1:

U87-MG Whole cell extract at 30 µg

Lane 2:

U87-MG Whole cell extract + conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human colon cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human lung cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human breast carcinoma tissue stained for MMP2 protein at cytosol and nucleus with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB97779)

Immunofluorescence analysis of 4% paraformaldehyde fixed U87-MG cells labelling MMP2 protein at cytoplasm using ab97779 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker was stained with anti-alpha Tubulin antibody at a 1/1000 dilution (Red). The nuclear counter stain is DAPI (Blue).

• IP

Supplier Data

Immunoprecipitation - Anti-MMP2 antibody (AB97779)

MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 μg ab97779. Western blot was performed from the immunoprecipitate using ab97779.

Lane 1 : Control IgG IP in U-87 MG whole cell extracts.

Lane 2 : ab97779 IP in U-87 MG whole cell extracts.

All lanes:

Immunoprecipitation - Anti-MMP2 antibody (ab97779)

Predicted band size: 73 kDa

false

• WB

Supplier Data

Western blot - Anti-MMP2 antibody (AB97779)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution

All lanes:

U87-MG Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false

• WB

Supplier Data

Western blot - Anti-MMP2 antibody (AB97779)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution

All lanes:

Mouse heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

false