Anti-MMP2 antibody (ab97779) is a rabbit polyclonal antibody that is used to detect MMP2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse samples.
- Over 190 publications
- Trusted since 2010
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- Journal of ovarian research 16:2122023Baicalein improves the chemoresistance of ovarian cancer through regulation of CirSLC7A6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuqing Li,Zhihui Yi,Mingqing Li,Zhiling ZhuPubMed 37940982
- iScience 26:1082722023DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoya Zhang,Xinhui Du,Yichao Fan,Guoxin Qu,Lon Kai Pang,Ruiying Zhao,Weitao YaoPubMed 38026218
- Mediators of inflammation 2023:61123012023Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBysani Chandrasekar,Srinivas Mummidi,Vincent G DeMarco,Yusuke HigashiPubMed 37830075
- Discover. Oncology 14:802023NEK2 promotes esophageal squamous cell carcinoma cell proliferation, migration and invasion through the Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Guo,Weinan Yao,Xingyu Du,Jing Dong,Xueyuan Zhang,Wenbin Shen,Shuchai ZhuPubMed 37233832
- Translational lung cancer research 12:1062-10772023Harmine loaded Au@MSNs@PEG@Asp6 nano-composites for treatment of spinal metastasis from lung adenocarcinoma by targeting ANXA9 experiment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoulei Wang,Fancheng Chen,Annan Hu,Haifeng Liang,Yun Liang,Nagashree Seetharamu,Huiren Wang,Jian DongPubMed 37323183
- Functional & integrative genomics 23:1642023IL-1RA inhibits esophageal carcinogenesis and lymphangiogenesis via downregulating VEGF-C and MMP9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Shen,Peipei Zhang,Weiguang Zhang,Fei Luo,Hui Xu,Shuchen Chen,Mingqiang KangPubMed 37198330
- Journal of Cancer 14:1362-13702023MiR-363 restrain the proliferation, migration and invasion of colorectal carcinoma cell by targeting E2F3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Yang,Xiqian Yang,Yang Zhang,Yunhai Li,Wenliang LvPubMed 37283793
- Molecular & cellular proteomics : MCP 22:1005232023Cerebrospinal Fluid Protein Markers Indicate Neuro-Damage in SARS-CoV-2-Infected Nonhuman Primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSudipa Maity,Meredith G Mayer,Qingbo Shu,Hellmers Linh,Duran Bao,Robert V Blair,Yanlin He,Christopher J Lyon,Tony Y Hu,Tracy Fischer,Jia FanPubMed 36870567
- Physiological reports 11:e155812023Interleukin-38 suppresses abdominal aortic aneurysm formation in mice by regulating macrophages in an IL1RL2-p38 pathway-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShun Kurose,Yutaka Matsubara,Shinichiro Yoshino,Keiji Yoshiya,Koichi Morisaki,Tadashi Furuyama,Tomoaki Hoshino,Tomoharu YoshizumiPubMed 36708509
- BMC cancer 22:10442022Lnc-SELPLG-2:1 enhanced osteosarcoma oncogenesis via hsa-miR-10a-5p and the BTRC cascade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyuan Li,Ming Zeng,Lin Yang,Jianshao Tan,Jianqi Yang,Hongye Guan,Manyuan Kuang,Jiaying LiPubMed 36199080
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMP2 aa 150-550. The exact immunogen used to generate this antibody is proprietary information. Database link P08253
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Alternative names
CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2
Recommended products
Anti-MMP2 antibody (ab97779) is a rabbit polyclonal antibody that is used to detect MMP2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse samples.
- Over 190 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMP2 aa 150-550. The exact immunogen used to generate this antibody is proprietary information. Database link P08253
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MMP2 antibody (ab97779) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of MMP2?
Anti-MMP2 (ab97779) specifically detects a band for MMP2 (UniProt: P08253) at a molecular weight of 74kDa.
Trusted by the scientific community
Anti-MMP2 (ab97779) was first used in a scientific publication in 2010 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-MMP2 (ab97779) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
- Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
- Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
- Associated diseases and disorders
Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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12 product images
Western blot - Anti-MMP2 antibody (ab97779)
All lanes: Western blot - Anti-MMP2 antibody (ab97779) at 1/3000 dilution
Lane 1: PC-3 at 30 µg
Lane 2: U-87 MG at 30 µg
Lane 3: SK-N-SH at 30 µg
Predicted band size: 73 kDa
Western blot - Anti-MMP2 antibody (ab97779)
7.5% SDS Page
All lanes: Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.
Immunoprecipitation - Anti-MMP2 antibody (ab97779)
MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 μg ab97779. Western blot was performed from the immunoprecipitate using ab97779.
Lane 1: Control IgG IP in U-87 MG whole cell extracts.
Lane 2: ab97779 IP in U-87 MG whole cell extracts.
All lanes: Immunoprecipitation - Anti-MMP2 antibody (ab97779)
Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.
Western blot - Anti-MMP2 antibody (ab97779)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution
All lanes: U87-MG Whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 73 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (ab97779)
Immunofluorescence analysis of 4% paraformaldehyde fixed U87-MG cells labelling MMP2 protein at cytoplasm using ab97779 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker was stained with anti-alpha Tubulin antibody at a 1/1000 dilution (Red). The nuclear counter stain is DAPI (Blue).
Western blot - Anti-MMP2 antibody (ab97779)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution
Lane 1: U87-MG Whole cell extract at 30 µg
Lane 2: U87-MG Whole cell extract + conditioned medium at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 73 kDa
Western blot - Anti-MMP2 antibody (ab97779)
Samples were seperated by 7.5% SDS-PAGE.
All lanes: Western blot - Anti-MMP2 antibody (ab97779) at 1/1000 dilution
All lanes: Mouse heart tissue extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
Paraffin-embedded human lung cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
Paraffin-embedded human colon cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (ab97779)
Paraffin-embedded human breast carcinoma tissue stained for MMP2 protein at cytosol and nucleus with ab97779 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min.
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