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Anti-MMP2 antibody [EPR1184] is a rabbit recombinant monoclonal antibody that is used to detect MMP2 in Flow cytometry (Intra), ICC/IF, Western blot. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 370 publications


Images

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR1184] (AB92536), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR1184] (AB92536), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] (AB92536), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR1184] (AB92536), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

For Lysate preparation protocol, please refer to the protocol here.

Compared with ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

For Lysate preparation protocol, please refer to the protocol here.

Compared with ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.

Species
Human
Dilution info
1/1000 - 1/5000
Notes

For Lysate preparation protocol, please refer to the protocol here.

Compared with ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

For unpurified, use 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

Function

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Alternative names

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

Recommended products

Anti-MMP2 antibody [EPR1184] is a rabbit recombinant monoclonal antibody that is used to detect MMP2 in Flow cytometry (Intra), ICC/IF, Western blot. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 370 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1184
Purification technique
Affinity purification Protein A
Specificity

In Western Blot, this product typically gives a weaker signal in some hepatocarcinoma like HepG2, MHCC97L(PMID: 33184263 ) et al. Please use reconmended positive control when testing these cells.
Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Product Specifications
Anti-MMP2 antibody [EPR1184] (ab92536) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, WB in human, mouse, rat samples.
Anti-MMP2 antibody [EPR1184] (ab92536) specifically detects MMP2 (UniProt ID: P08253; Molecular weight: 62kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MMP2 antibody [EPR1184] (ab92536) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MMP2 antibody [EPR1184] (ab92536) has been cited over 376 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP2 antibody [EPR1184] (ab92536) has 7 independent reviews from customers.

Related Products
Antibody clone EPR1184 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, APC, PE, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555 (Alexa Fluor® 488 Anti-MMP2 antibody [EPR1184] ab237473, Alexa Fluor® 647 Anti-MMP2 antibody [EPR1184] ab237474, APC Anti-MMP2 antibody [EPR1184] ab310884, PE Anti-MMP2 antibody [EPR1184] ab310940, Alexa Fluor® 594 Anti-MMP2 antibody [EPR1184] ab311718, Alexa Fluor® 568 Anti-MMP2 antibody [EPR1184] ab312994, Alexa Fluor® 555 Anti-MMP2 antibody [EPR1184] ab313199).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.

    Compared with ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.

    Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution

    Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR17003-25] (Anti-MMP2 antibody [EPR17003-25] ab181286)

    Lane 1: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg

    Lane 4: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 73 kDa

    Exposure time: 60s

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    MMP2 Western blot staining using rabbit Anti-MMP2 antibody

    The 72 KDa band is pro-MMP2 and the 69 KDa band is active-MMP2 reported by PMID 11489818 and 22190701.

    This antibody shows low affinity in detecting mouse liver and HepG2 lysates which are positive for MMP2 reported by PMID 24096707 and 24297510.

    All lanes: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution

    Lane 1: L6 (Rat skeletal muscle myoblast) whole cell lysates prepared in 1%SDS Hot lysis method at 20 µg

    Lane 2: Mouse liver lysates prepared in RIPA lysis method at 20 µg

    Lane 3: Mouse liver lysates prepared in 1%SDS Hot lysis method at 20 µg

    Lane 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared in RIPA lysis method at 20 µg

    Lane 5: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared in 1%SDS Hot lysis method at 20 µg

    Lane 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 20 µg

    Lane 7: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa

    Observed band size: 69 kDa, 72 kDa

    Exposure time: 10s

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    72kDa: propeptide; 64kDa: active form

    All lanes: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/10000 dilution

    Lane 1: L6 cell lysate at 20 µg

    Lane 2: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 73 kDa

    Observed band size: 64 kDa, 72 kDa

  • Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] (ab92536)

    Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    All lanes: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution

    Lane 1: L6 cell lysate at 10 µg

    Lane 2: Fetal heart lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] (ab92536)

    Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    72kDa: propeptide; 64kDa: active form

    All lanes: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/5000 dilution

    All lanes: human skin at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 73 kDa

    Observed band size: 64 kDa, 72 kDa

  • Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] (ab92536)

    Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified ab92536 at 1/180 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-MMP2 antibody [EPR1184] (ab92536), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR1184] (ab92536)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Compared with ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.

    Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR17003-25] (Anti-MMP2 antibody [EPR17003-25] ab181286)

    Lane 1: Human plasma tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Lane 3: Human breast tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 73 kDa

    Observed band size: 69 kDa, 72 kDa

    Exposure time: 60s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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