Rabbit Recombinant Monoclonal MMP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--flow-cytometry-intracellular-img111059.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866)")
![Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--immunocytochemistry-immunofluorescence-img54589.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866)")
![Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--flow-cytometry-intracellular-img415702.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866)")
![Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--western-blot-img418931.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866)")
![Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--western-blot-img418930.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (AB271866)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For Lysate preparation protocol, please refer to the protocol ;here (downloadable copy). Compared with Anti-MMP2 antibody [EPR1184] ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
4 products for Alternative Version
Target data
Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.Isoform 2Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Alternative names
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2, CLG4A, MMP2
Recommended products
Rabbit Recombinant Monoclonal MMP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2, CLG4A, MMP2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR1184
- Purification technique
Affinity purification Protein A
- Specificity
Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab271866 is the carrier-free version of Anti-MMP2 antibody [EPR1184] ab92536.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
- Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
- Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
- Associated diseases and disorders
Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--flow-cytometry-intracellular-img111059.jpg "Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)")
Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)
Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified Anti-MMP2 antibody [EPR1184] ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP2 antibody [EPR1184] ab92536).
![Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--immunocytochemistry-immunofluorescence-img54589.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)")
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)
Immunofluorescence staining of PC-3 cells with purified Anti-MMP2 antibody [EPR1184] ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-MMP2 antibody [EPR1184] ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP2 antibody [EPR1184] ab92536).
![Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--flow-cytometry-intracellular-img415702.jpg "Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)")
Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)
Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified Anti-MMP2 antibody [EPR1184] ab92536 at 1/180 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP2 antibody [EPR1184] ab92536).
![Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--western-blot-img418931.jpg "Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)")
Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP2 antibody [EPR1184] ab92536).
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.
Compared with Anti-MMP2 antibody [EPR1184] ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR1184] (Anti-MMP2 antibody [EPR1184] ab92536) at 1/1000 dilution
Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR17003-25] (Anti-MMP2 antibody [EPR17003-25] ab181286)
Lane 1: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg
Lane 4: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 73 kDa
Exposure time: 60s
![Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866), expandable thumbnail](https://content.abcam.com/products/images/mmp2-antibody-epr1184-bsa-and-azide-free-ab271866--western-blot-img418930.jpg "Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)")
Western blot - Anti-MMP2 antibody [EPR1184] - BSA and Azide free (ab271866)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP2 antibody [EPR1184] ab92536).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
Compared with Anti-MMP2 antibody [EPR1184] ab92536, Anti-MMP2 antibody [EPR17003-25] ab181286 has higher sensitivity. We recommend Anti-MMP2 antibody [EPR17003-25] ab181286 as an alternative for testing MMP2 in western blot.Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR1184] (Anti-MMP2 antibody [EPR1184] ab92536) at 1/1000 dilution
Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR17003-25] (Anti-MMP2 antibody [EPR17003-25] ab181286)
Lane 1: Human plasma tissue lysate at 20 µg
Lane 2: Human brain tissue lysate at 20 µg
Lane 3: Human breast tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 69 kDa, 72 kDa
Exposure time: 60s
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