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Rabbit Recombinant Monoclonal MMP2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 27 publications.

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Images

Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (AB181286), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286), expandable thumbnail
  • Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.Isoform 2Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MMP2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 27 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17003-25

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.

Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Associated diseases and disorders

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (ab181286), expandable thumbnail

    Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    MMP2 was immunoprecipitated from 0.35 mg HT1080 (human fibrosarcoma cell line) whole cell lysate with ab181286 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181286 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HT1080 whole cell lysate 10 μg (Input).
    Lane 2: ab181286 IP in HT1080 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181286 in HT1080 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Predicted band size: 73 kDa

  • Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Exposure time: Lanes 1-2: 3 minutes; Lanes 3-5: 10 seconds; Lanes 6-7: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/1000 dilution

    Lanes 6 - 7: Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/2000 dilution

    Lane 1: Mouse plasma lysate at 20 µg

    Lane 2: Rat serum lysate at 20 µg

    Lane 3: Mouse lung lysate at 20 µg

    Lane 4: Rat plasma lysate at 20 µg

    Lane 5: Rat lung lysate at 20 µg

    Lane 6: HT1080 (human fibrosarcoma cell line) whole cell lysate at 10 µg

    Lane 7: Human plasma lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 73 kDa

    Observed band size: 72 kDa

  • Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.

    Compared with Anti-MMP2 antibody [EPR1184] ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.

    Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR1184] (Anti-MMP2 antibody [EPR1184] ab92536) at 1/1000 dilution

    Lanes 1 - 5: Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Lane 1: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg

    Lane 4: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 73 kDa

    Exposure time: 60s

  • Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286), expandable thumbnail

    Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Compared with Anti-MMP2 antibody [EPR1184] ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.

    Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR1184] (Anti-MMP2 antibody [EPR1184] ab92536) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

    Lane 1: Human plasma tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Lane 3: Human breast tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 73 kDa

    Observed band size: 69 kDa, 72 kDa

    Exposure time: 60s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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