Anti-MMP2 antibody [EPR17003-25]

• WB

Supplier Data

Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286)

Exposure time : Lanes 1-2 : 3 minutes; Lanes 3-5 : 10 seconds; Lanes 6-7 : 30 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

Lanes 1 - 5:

Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/1000 dilution

Lanes 6 - 7:

Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286) at 1/2000 dilution

Lane 1:

Mouse plasma lysate at 20 µg

Lane 2:

Rat serum lysate at 20 µg

Lane 3:

Mouse lung lysate at 20 µg

Lane 4:

Rat plasma lysate at 20 µg

Lane 5:

Rat lung lysate at 20 µg

Lane 6:

HT1080 (human fibrosarcoma cell line) whole cell lysate at 10 µg

Lane 7:

Human plasma lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 72 kDa

false

• WB

Lab

Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as GAPDH loading control. Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.

Lanes 1 - 5:

Western blot - Anti-MMP2 antibody [EPR1184] (<a href='/en-us/products/primary-antibodies/mmp2-antibody-epr1184-ab92536'>ab92536</a>) at 1/1000 dilution

Lanes 1 - 5:

Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

Lane 1:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg

Lane 4:

Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 73 kDa

false

Exposure time: 60s

• WB

Lab

Western blot - Anti-MMP2 antibody [EPR17003-25] (AB181286)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.

Lanes 1 - 3:

Western blot - Anti-MMP2 antibody [EPR1184] (<a href='/en-us/products/primary-antibodies/mmp2-antibody-epr1184-ab92536'>ab92536</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-MMP2 antibody [EPR17003-25] (ab181286)

Lane 1:

Human plasma tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Human breast tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 69 kDa,72 kDa

false

Exposure time: 60s

• IP

Unknown

Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (AB181286)

MMP2 was immunoprecipitated from 0.35 mg HT1080 (human fibrosarcoma cell line) whole cell lysate with ab181286 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181286 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HT1080 whole cell lysate 10 μg (Input).
Lane 2 : ab181286 IP in HT1080 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181286 in HT1080 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (ab181286)

Predicted band size: 73 kDa

false