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AB76109

Anti-MMP20 antibody [EP1275Y]

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(4 Publications)

Rabbit Recombinant Monoclonal MMP20 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Matrix metalloproteinase-20, MMP-20, Enamel metalloproteinase, Enamelysin, MMP20

1 Images
Western blot - Anti-MMP20 antibody [EP1275Y] (AB76109)
  • WB

Lab

Western blot - Anti-MMP20 antibody [EP1275Y] (AB76109)

Exposure time : Lane 1 and Lane 2 : 20 seconds, Lane 3 : 3 seconds

All lanes:

Western blot - Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 15 µg with 5% NFDM/TBST

Lane 2:

Mouse heart lysate at 15 µg with 5% NFDM/TBST

Lane 3:

Rat heart lysate at 15 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1275Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP20 also known as Enamelysin is a member of the matrix metalloproteinase (MMP) family. This enzyme has a molecular mass of approximately 54 kDa. MMP20 is mainly expressed in the dental enamel but researchers have also observed its expression in other tissues like the epithelial and mesenchymal cells of the developing tooth. The enzyme plays a role in the breakdown of extracellular matrix components specifically during the formation of tooth enamel where it processes the nascent enamel matrix proteins.
Biological function summary

MMP20 participates in enamel development and maturation. This enzyme cleaves amelogenin an important protein in the enamel matrix to aid in proper enamel mineralization. MMP20 does not function alone; it exists as part of the complex processes necessary for the tooth structure formation. By regulating protein processing MMP20 ensures the replacement of organic matrix with inorganic mineral important for the hardness of enamel.

Pathways

The involvement of MMP20 is critical in the enamel mineralization pathway and intralays network of amelogenesis. It connects with other proteins like ameloblastin and enamelin which collaborate in enamel formation. MMP20 affects these pathways by enabling the proteolytic processing of enamel-specific proteins supporting the structural changes and mineral deposition essential for hard enamel formation.

The role of MMP20 appears significant in amelogenesis imperfecta a genetic condition that affects enamel formation. Mutations within MMP20 can lead to defects in enamel structure causing increased susceptibility to tooth decay and damage. Furthermore MMP20 interacts with other proteins involved in enamel anomalies like amelogenin aggrevating the condition's manifestations when dysfunction occurs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix : aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.
See full target information MMP20
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 10:139 PubMed31109359

2019

Effect of canonical NF-κB signaling pathway on the differentiation of rat dental epithelial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Guoqing Chen,Yuzhi Yang,Ziyue Li,Tian Chen,Wenhua Sun,Mei Yu,Kuangwu Pan,Weihua Guo,Weidong Tian

Molecular medicine reports 15:297-304 PubMed27959403

2016

Bcl11b regulates enamel matrix protein expression and dental epithelial cell differentiation during rat tooth development.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyue Li,Guoqing Chen,Yaling Yang,Weihua Guo,Weidong Tian

Archives of oral biology 58:1165-73 PubMed23598055

2013

High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2.

Applications

WB

Species

Mouse

Ting Yang,Yanli Zhang,Yanling Li,Ying Hao,Mi Zhou,Nan Dong,Xiaohong Duan

Journal of cell science 126:732-44 PubMed23264742

2012

Critical role for αvβ6 integrin in enamel biomineralization.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Mohazab,Leeni Koivisto,Guoqiao Jiang,Leena Kytömäki,Markus Haapasalo,Gethin R Owen,Colin Wiebe,Yanshuang Xie,Kristiina Heikinheimo,Toshiyuki Yoshida,Charles E Smith,Jyrki Heino,Lari Häkkinen,Marc D McKee,Hannu Larjava
View all publications
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Product promise

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