AB250359

Anti-MMP23 antibody [EPR7769] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal MMP23 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MMP21, MMP22, MMP23B, Matrix metalloproteinase-23, MMP-23, Femalysin, MIFR-1, Matrix metalloproteinase-21, Matrix metalloproteinase-22, MMP-21, MMP-22

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

This data was developed using ab181118, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP23 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

This data was developed using ab181118, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP23 with ab181118 at 1/250. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

This data was developed using ab181118, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MMP23 antibody [EPR7769] (<a href='/en-us/products/primary-antibodies/mmp23-antibody-epr7769-ab181118'>ab181118</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 44 kDa

Observed band size: 56 kDa

false

Supplier Data

Western blot - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

This data was developed using ab181118, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MMP23 antibody [EPR7769] (<a href='/en-us/products/primary-antibodies/mmp23-antibody-epr7769-ab181118'>ab181118</a>) at 1/1000 dilution

Lane 1:

Calu 3 cell lysate at 10 µg

Lane 2:

SK OV 3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 56 kDa

false

Unconjugated

Anti-MMP23 antibody [EPR7769]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7769

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab250359 is the carrier-free version of ab181118.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MMP23 also known as matrix metalloproteinase-23 plays a role in tissue remodeling and cellular processes. MMP23 is unique due to its membrane-type structure distinguishing it from other MMP family members. The protein weighs approximately 44 kDa. MMP23 is mainly found in a variety of tissues including the testis ovary and placenta but its expression is notably less widespread compared to other metalloproteinases.

Biological function summary

Matrix metalloproteinase-23 contributes to modulating extracellular matrix degradation. Unlike some MMPs MMP23 is part of a transmembrane complex impacting localized tissue environments. It influences cellular growth and communication through its regulatory effects on the extracellular space. Such interactions play a part in processes like cell migration and signal transduction important for normal physiological and developmental activities.

Pathways

MMP23 participates in specific biochemical pathways important for tissue repair and immune response. One significant pathway is the extracellular matrix (ECM) remodeling pathway where MMP23 interacts with other metalloproteinases such as MMP2. Additionally MMP23's activity affects signaling pathways by altering the ECM components which can influence cellular behaviors linked to proliferation and apoptosis.

Matrix metalloproteinase-23 implicates its role in certain pathologies including cancer and inflammatory diseases. Its ability to modify the extracellular matrix associates it with tumor invasion and metastasis particularly through interactions with proteins like TIMP (tissue inhibitors of metalloproteinases). Inflammatory conditions also show altered MMP23 activity indicating its potential involvement in chronic inflammation pathophysiology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).

See full target information MMP23B

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP23 antibody [EPR7769] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

Western blot - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

Western blot - Anti-MMP23 antibody [EPR7769] - BSA and Azide free (AB250359)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise