AB150415

Anti-MMP27 antibody [EPR7543]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MMP27 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ2503/PRO5992, MMP27, Matrix metalloproteinase-27, MMP-27

1 Images

Unknown

Western blot - Anti-MMP27 antibody [EPR7543] (AB150415)

All lanes:

Western blot - Anti-MMP27 antibody [EPR7543] (ab150415) at 1/1000 dilution

Lane 1:

Caco-1 cell lysate at 10 µg

Lane 2:

Human fetal lung cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 59 kDa

false

Carrier free

Anti-MMP27 antibody [EPR7543] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7543

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP27 also known as matrix metallopeptidase 27 is part of the matrix metalloproteinases (MMPs) family. This protein has a molecular mass of approximately 54 kDa. MMPs are known for their role in cleaving extracellular matrix components. MMP27 expresses in several human tissues including lymphoid organs like the spleen and lymph nodes. It involves in processes related to tissue remodeling and repair as well as immune functions.

Biological function summary

MMP27 participates in the breakdown of proteins in the extracellular matrix. It does this as a monomer unlike some other metalloproteinases that form complexes. Although its specific substrate preferences are not completely characterized MMP27 likely interacts with proteoglycans and collagen influencing the structural components of cells and tissues. This enables various cell migration and differentiation events.

Pathways

Enzymes of the MMP family like MMP27 play roles in the connective tissue remodeling pathway and the immune response regulation pathway. In these pathways MMP27 interacts with other proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity. The balance between MMPs and TIMPs is vital for maintaining tissue homeostasis and proper immune system function.

Irregular expression or activity of MMP27 can relate to disorders like cancer and rheumatoid arthritis. MMP27’s role in degrading extracellular matrix components affects tumor progression and inflammation. In cancer it may interact with other MMPs such as MMP9 facilitating metastasis. In rheumatoid arthritis MMP27 could contribute to joint inflammation and destruction of cartilage interacting with cytokines that modulate inflammatory responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Matrix metalloproteinases degrade protein components of the extracellular matrix such as fibronectin, laminin, gelatins and/or collagens.

See full target information MMP27

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 115:E9687-E9696 PubMed30254165

2018

Blood-derived plasminogen drives brain inflammation and plaque deposition in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Baker,Zu-Lin Chen,Erin H Norris,Alexey S Revenko,A Robert MacLeod,Sidney Strickland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP27 antibody [EPR7543]

Western blot - Anti-MMP27 antibody [EPR7543] (AB150415)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise