AB175937

Anti-MMP28 antibody [EP1341(2)]

RabMAb
What is this?

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(3 Publications)

Rabbit Monoclonal MMP28 antibody. Suitable for WB and reacts with Recombinant fragment, Human samples. Cited in 3 publications.

View Alternative Names

MMP25, UNQ1893/PRO4339, MMP28, Matrix metalloproteinase-28, MMP-28, Epilysin

2 Images

Supplier Data

Western blot - Anti-MMP28 antibody [EP1341(2)] (AB175937)

All lanes:

Western blot - Anti-MMP28 antibody [EP1341(2)] (ab175937) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Predicted band size: 59 kDa

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Supplier Data

Western blot - Anti-MMP28 antibody [EP1341(2)] (AB175937)

All lanes:

Western blot - Anti-MMP28 antibody [EP1341(2)] (ab175937) at 1/1000 dilution

All lanes:

MMP-28 recombinant protein fragment at 0.01 µg

Predicted band size: 59 kDa

false

Carrier free

Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1341(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP28 also known as epilysin is a member of the matrix metalloproteinase family. It weighs approximately 53 kDa. This protein is primarily expressed in skin keratinocytes placenta and testis tissues. MMP28 functions mechanically as a protease responsible for the degradation of extracellular matrix components. Its regulation can impact tissue remodeling wound repair and maintenance of homeostasis in the skin and other tissues.

Biological function summary

Matrix metalloproteinase-28 (MMP28) contributes to the dynamic restructuring of tissues. Epilysin is not known to be part of a protein complex but interacts closely with other matrix components for effective extracellular matrix modulation. It helps regulate cell migration and proliferation by modifying the surrounding extracellular environment ensuring the precise orchestration of cellular behaviors during developmental and healing processes.

Pathways

The activity of MMP28 aligns importantly with the extracellular matrix degradation pathway and tissue remodeling pathway. Within these pathways MMP28 interacts with other matrix metalloproteinases such as MMP2 and MMP9. Together they participate in breaking down collagen and other matrix proteins facilitating necessary structural alterations in tissues during various biological events.

Researchers have observed MMP28’s involvement in several pathological conditions such as fibrosis and cancer. In the context of fibrosis MMP28's activity relates to irregular tissue remodeling potentially contributing to fibrotic transformation in organs. In cancer elevated MMP28 expression correlates with tumor progression and metastasis where it interacts with collagens and other extracellular matrix components enabling tumor cells to invade and spread. Insights into MMP28's relationships with other proteins like MMP9 can help develop therapeutic strategies targeting its dysregulation in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Can degrade casein. Could play a role in tissues homeostasis and repair.

See full target information MMP28

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed35008415

2022

Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression.

Applications

Unspecified application

Species

Unspecified reactive species

James Drury,Piotr G Rychahou,Courtney O Kelson,Mariah E Geisen,Yuanyuan Wu,Daheng He,Chi Wang,Eun Y Lee,B Mark Evers,Yekaterina Y Zaytseva

International journal of molecular sciences 22: PubMed33923282

2021

Matrix Metalloproteinases and Their Role in Mechanisms Underlying Effects of Quercetin on Heart Function in Aged Zucker Diabetic Fatty Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Barbora Boťanská,Monika Barteková,Kristína Ferenczyová,Mária Fogarassyová,Lucia Kindernay,Miroslav Barančík

Cancer medicine 7:2485-2503 PubMed29663730

2018

MiR-21 improves invasion and migration of drug-resistant lung adenocarcinoma cancer cell and transformation of EMT through targeting HBP1.

Applications

Species

Human

Chongyu Su,Xu Cheng,Yunsong Li,Yi Han,Xiaoyun Song,Daping Yu,Xiaoqing Cao,Zhidong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP28 antibody [EP1341(2)]

Western blot - Anti-MMP28 antibody [EP1341(2)] (AB175937)

Western blot - Anti-MMP28 antibody [EP1341(2)] (AB175937)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise