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AB284827

Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free

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Rabbit Monoclonal MMP28 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein, Human samples.

View Alternative Names

MMP25, UNQ1893/PRO4339, MMP28, Matrix metalloproteinase-28, MMP-28, Epilysin

2 Images
Western blot - Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free (AB284827)
  • WB

Supplier Data

Western blot - Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free (AB284827)

This data was developed using ab175937, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MMP28 antibody [EP1341(2)] (<a href='/en-us/products/primary-antibodies/mmp28-antibody-ep13412-ab175937'>ab175937</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Predicted band size: 59 kDa

false

Western blot - Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free (AB284827)
  • WB

Supplier Data

Western blot - Anti-MMP28 antibody [EP1341(2)] - BSA and Azide free (AB284827)

This data was developed using ab175937, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MMP28 antibody [EP1341(2)] (<a href='/en-us/products/primary-antibodies/mmp28-antibody-ep13412-ab175937'>ab175937</a>) at 1/1000 dilution

All lanes:

MMP-28 recombinant protein fragment at 0.01 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1341(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284827 is the carrier-free version of ab175937

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP28 also known as epilysin is a member of the matrix metalloproteinase family. It weighs approximately 53 kDa. This protein is primarily expressed in skin keratinocytes placenta and testis tissues. MMP28 functions mechanically as a protease responsible for the degradation of extracellular matrix components. Its regulation can impact tissue remodeling wound repair and maintenance of homeostasis in the skin and other tissues.
Biological function summary

Matrix metalloproteinase-28 (MMP28) contributes to the dynamic restructuring of tissues. Epilysin is not known to be part of a protein complex but interacts closely with other matrix components for effective extracellular matrix modulation. It helps regulate cell migration and proliferation by modifying the surrounding extracellular environment ensuring the precise orchestration of cellular behaviors during developmental and healing processes.

Pathways

The activity of MMP28 aligns importantly with the extracellular matrix degradation pathway and tissue remodeling pathway. Within these pathways MMP28 interacts with other matrix metalloproteinases such as MMP2 and MMP9. Together they participate in breaking down collagen and other matrix proteins facilitating necessary structural alterations in tissues during various biological events.

Researchers have observed MMP28’s involvement in several pathological conditions such as fibrosis and cancer. In the context of fibrosis MMP28's activity relates to irregular tissue remodeling potentially contributing to fibrotic transformation in organs. In cancer elevated MMP28 expression correlates with tumor progression and metastasis where it interacts with collagens and other extracellular matrix components enabling tumor cells to invade and spread. Insights into MMP28's relationships with other proteins like MMP9 can help develop therapeutic strategies targeting its dysregulation in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can degrade casein. Could play a role in tissues homeostasis and repair.
See full target information MMP28
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com