AB137659

Anti-MMP3 antibody

Name: Anti-MMP3 antibody (ab137659) | Abcam
Brand: Abcam Limited
SKU: AB137659
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal MMP3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MMP3 aa 200-350.

View Alternative Names

STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (AB137659)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (AB137659)

Immunofluorescence analysis of methanol-fixed HeLa cells labelling MMP3 with ab137659 at 1/200 dilution. Lower panel is costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (AB137659)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (AB137659)

Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling MMP3 with ab137659 at 1/500 dilution.

Unknown

Western blot - Anti-MMP3 antibody (AB137659)

10% SDS PAGE

All lanes:

Western blot - Anti-MMP3 antibody (ab137659) at 1/500 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human MMP3 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

P08254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.

Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed : 11029580, PubMed : 1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed : 2383557). Acts also intracellularly (PubMed : 22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).

See full target information MMP3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:186 PubMed36894989

2023

MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchun Zhu,Chi Zhang,Bo Jiang,Qirong Dong

Oxidative medicine and cellular longevity 2022:7655142 PubMed35265264

2022

Naringin protects human nucleus pulposus cells against TNF--induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Renchang Chen,Shang Gao,Huapeng Guan,Xin Zhang,Yuliang Gao,Youxiang Su,Yun Song,Yuehua Jiang,Nianhu Li

IUBMB life 73:92-107 PubMed33159503

2020

Soy isoflavone genistein inhibits hsa_circ_0031250/miR-873-5p/FOXM1 axis to suppress non-small-cell lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yaying Yu,Yanwei Xing,Qian Zhang,Qianqian Zhang,Shuangjian Huang,Xinxin Li,Chao Gao

Cancer management and research 11:5845-5856 PubMed31388313

2019

MicroRNA-3662 targets ZEB1 and attenuates the invasion of the highly aggressive melanoma cell line A375.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Zhifei Liu,Ruijia Dong,Xiaojun Wang,Mingzi Zhang,Xiao Guo,Nanze Yu,Ang Zeng

The Journal of pathology 244:311-322 PubMed29210073

2018

Interleukin-17 regulates matrix metalloproteinase activity in human pulmonary tuberculosis.

Applications

IHC-P

Species

Human

Shivani Singh,George Maniakis-Grivas,Utpal K Singh,Radha M Asher,Francesco Mauri,Paul T Elkington,Jon S Friedland

Clinical sarcoma research 6:10 PubMed27358721

2016

Phosphorylated-insulin growth factor I receptor (p-IGF1R) and metalloproteinase-3 (MMP3) expression in advanced gastrointestinal stromal tumors (GIST). A GEIS 19 study.

Applications

Unspecified application

Species

Unspecified reactive species

Joan Maurel,Antonio López-Pousa,Silvia Calabuig,Silvia Bagué,Xavier Garcia Del Muro,Xavier Sanjuan,Jordi Rubió-Casadevall,Miriam Cuatrecasas,Javier Martinez-Trufero,Carlos Horndler,Joaquin Fra,Claudia Valverde,Andrés Redondo,Andrés Poveda,Isabel Sevilla,Nuria Lainez,Michele Rubini,Xabier García-Albéniz,Javier Martín-Broto,Enrique de Alava

Molecular medicine reports 12:1829-37 PubMed25847066

2015

Effect of β-anhydroicaritin on the expression levels of tumor necrosis factor-α and matrix metalloproteinase-3 in periodontal tissue of diabetic rats.

Applications

Unspecified application

Species

Rat

Yingtao Wu,Wanchun Wang,Lian Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MMP3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody (AB137659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody (AB137659)

Western blot - Anti-MMP3 antibody (AB137659)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise