Name: Anti-MMP3 antibody [EP1186Y]
SKU: ab52915
Rating: 5 (4 reviews)

Anti-MMP3 antibody [EP1186Y] is a rabbit monoclonal antibody that is used in MMP3 western blot (WB), IHC and immunofluorescence (ICC/IF). Suitable for human, mouse and rat samples.

- Antibody clone EP1186Y is the most widely used clone for MMP3 on the market, cited in over 225 publications
- Specificity confirmed with MMP3 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Affinity-purified using Protein A

Related conjugates and formulations

ab194980
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Alexa Fluor® 488 Anti-MMP3 antibody [EP1186Y]
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Alexa Fluor® 555 Anti-MMP3 antibody [EP1186Y]
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Alexa Fluor® 594 Anti-MMP3 antibody [EP1186Y]
ab194717
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Alexa Fluor® 647 Anti-MMP3 antibody [EP1186Y]
ab214794
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Anti-MMP3 antibody [EP1186Y] - BSA and Azide free
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Alexa Fluor® 568 Anti-MMP3 antibody [EP1186Y]
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Alexa Fluor® 750 Anti-MMP3 antibody [EP1186Y]

Images

Western blot - Anti-MMP3 antibody [EP1186Y] (AB52915), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IHC-P
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/20000	Notes -
Species Rat	Dilution info 1/1000 - 1/20000	Notes -
Species Human	Dilution info 1/1000 - 1/20000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/50	Notes We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/50	Notes We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information MMP3

Function

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed:11029580, PubMed:1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed:2383557). Acts also intracellularly (PubMed:22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed:21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed:35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed:35940311).

Alternative names

STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3

Recommended products

Anti-MMP3 antibody [EP1186Y] is a rabbit monoclonal antibody that is used in MMP3 western blot (WB), IHC and immunofluorescence (ICC/IF). Suitable for human, mouse and rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1186Y
Purification technique: Affinity purification Protein A
Specificity: 79% identities with MMP10
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-MMP3 antibody [EP1186Y] (ab52915) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-MMP3 antibody [EP1186Y] (ab52915) specifically detects MMP3 (UniProt ID: P08254; Molecular weight: 43kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MMP3 antibody [EP1186Y] (ab52915) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-MMP3 antibody [EP1186Y] (ab52915) has been confirmed by testing in knockout samples.
Anti-MMP3 antibody [EP1186Y] (ab52915) has been cited over 230 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP3 antibody [EP1186Y] (ab52915) has 4 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EP1186Y - ab216656.
Antibody clone EP1186Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750, (ab194717, ab194980, ab311769, ab313050, ab313251, ab321671).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/mL
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate at 20 µg
Lane 3: Mouse lung lysate at 20 µg
Lane 4: Mouse placenta lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Exposure time: 84s
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1 & 2: 10 seconds
Lane 3: 3 minutes

All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/mL
Lane 1: Rat brain lysate at 20 µg
Lane 2: Rat liver lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)
Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes
Lane 2 & 3: 4 seconds
All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/mL
Lane 1: Mouse brain lysate at 20 µg
Lane 2: Mouse kidney lysate at 20 µg
Lane 3: Mouse liver lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)
Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Blocking and diluting buffer: 5% NFDM/TBST

All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/mL
All lanes: Rat kidney lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Exposure time: 15s
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution
All lanes: MMP3 recombinant protein
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)
Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)
Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)
Western blot: Anti-MMP3 antibody [EP1186Y] (ab52915) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab52915 was shown to bind specifically to MMP3. A band was observed at 55-60 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in MMP3 knockout cell line. To generate this image, wild-type and MMP3 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/1000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: MMP3 knockout U-87 MG cell lysate at 20 µg
Lane 3: Wild-type HeLa Treated: IL-1beta (10 ng/mL, 48 h) cell lysate at 20 µg
Lane 4: Wild-type HeLa Vehicle control: IL-1beta (0 ng/mL, 48 h) cell lysate at 20 µg
Lane 5: HeLa cell lysate at 20 µg
Lane 6: MCF7 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 55 kDa, 60 kDa