Anti-MMP3 antibody [EP1186Y] - BSA and Azide free

5 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] - BSA and Azide free (ab214794)

  Immunohistochemical staining of paraffin embedded rat kidney with purified Anti-MMP3 antibody [EP1186Y] ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP3 antibody [EP1186Y] ab52915).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] - BSA and Azide free (ab214794)

  Immunohistochemical staining of paraffin embedded mouse liver with purified Anti-MMP3 antibody [EP1186Y] ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MMP3 antibody [EP1186Y] ab52915).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] - BSA and Azide free (ab214794)

  This IHC data was generated using the same anti-MMP3 antibody clone, EP1186Y, in a different buffer formulation (cat# Anti-MMP3 antibody [EP1186Y] ab52915).
  

  Immunohistochemical staining of paraffin embedded human liver with purified Anti-MMP3 antibody [EP1186Y] ab52915 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

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  Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] - BSA and Azide free (ab214794)

  This ICC data was generated using the same anti-MMP3 antibody clone, EP1186Y, in a different buffer formulation (cat# Anti-MMP3 antibody [EP1186Y] ab52915).
  

  Immunofluorescence staining of HT-29 cells with purified Anti-MMP3 antibody [EP1186Y] ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-MMP3 antibody [EP1186Y] ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

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  Western blot - Anti-MMP3 antibody [EP1186Y] - BSA and Azide free (ab214794)

  This data was developed using the same antibody clone in a different buffer formulation (Anti-MMP3 antibody [EP1186Y] ab52915).

  Western blot: Anti-MMP3 antibody [EP1186Y] (Anti-MMP3 antibody [EP1186Y] ab52915) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-MMP3 antibody [EP1186Y] ab52915 was shown to bind specifically to MMP3. A band was observed at 55-60 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in MMP3 knockout cell line. To generate this image, wild-type and MMP3 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  All lanes: Western blot - Anti-MMP3 antibody [EP1186Y] (Anti-MMP3 antibody [EP1186Y] ab52915) at 1/1000 dilution

  Lane 1: Wild-type U-87 MG cell lysate at 20 µg

  Lane 2: MMP3 knockout U-87 MG cell lysate at 20 µg

  Lane 3: Wild-type HeLa Treated: IL-1beta (10 ng/mL, 48 h) cell lysate at 20 µg

  Lane 4: Wild-type HeLa Vehicle control: IL-1beta (0 ng/mL, 48 h) cell lysate at 20 µg

  Lane 5: HeLa cell lysate at 20 µg

  Lane 6: MCF7 cell lysate at 20 µg

  Secondary

  All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

  Performed under reducing conditions.

  Predicted band size: 53 kDa

  Observed band size: 55 kDa, 60 kDa