Anti-MMP7 antibody [EPR1251(2)]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MMP7 antibody [EPR1251(2)] (AB176325)

Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labeling MMP7using ab176325 at a 1/100 dilution (red) or a rabbit IgGcontrol (green).

• WB

Supplier Data

Western blot - Anti-MMP7 antibody [EPR1251(2)] (AB176325)

All lanes:

Western blot - Anti-MMP7 antibody [EPR1251(2)] (ab176325) at 1/1000 dilution

Lane 1:

Human prostate lysate at 10 µg

Lane 2:

BxPC-3 lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Predicted band size: 29 kDa

false

• WB

Collaborator

Western blot - Anti-MMP7 antibody [EPR1251(2)] (AB176325)

ab176325 was shown to react with MMP7 in wild-type A549 cells in Western blot with loss of signal observed in a MMP7 knockout cell line. Wild-type A549 and MMP7 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab176325 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-MMP7 antibody [EPR1251(2)] (ab176325) at 1/1000 dilution

Lane 1:

Wild-type HepG2 lysate at 30 µg

Lane 2:

MMP7 knock-out A549 lysate at 30 µg

false