Anti-MMP7 antibody [EPR17888-101]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human gastric carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Immunohistochemical analysis of paraffin-embedded Human prostatic carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human prostatic carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/2000 dilution

Lane 1:

A549 (Human lung carcinoma) whole cell lysate at 20 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution

All lanes:

Human prostate lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 5s

• WB

Collaborator

Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525)

ab205525 was shown to react with MMP7 in wild-type A549 cells in Western blot with loss of signal observed in a MMP7 knockout cell line. Wild-type A549 and MMP7 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab205525 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution

Lane 1:

Wild-type A549 lysate at 30 µg

Lane 2:

MMP7 knock-out A549 lysate at 30 µg

false