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Rabbit Recombinant Monoclonal MMP7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 26 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (AB205525), expandable thumbnail
  • Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525), expandable thumbnail
  • Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525), expandable thumbnail
  • Western blot - Anti-MMP7 antibody [EPR17888-101] (AB205525), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (AB205525), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

Alternative names

MPSL1, PUMP1, MMP7, Matrilysin, Matrin, Matrix metalloproteinase-7, Pump-1 protease, Uterine metalloproteinase, MMP-7

Recommended products

Rabbit Recombinant Monoclonal MMP7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 26 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17888-101
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MMP7 also known as matrilysin is a member of the matrix metalloproteinase family and has a molecular weight of approximately 28 kDa. Mechanically MMP7 participates in the breakdown of extracellular matrix components such as elastin casein and various collagens. It is important in tissue remodeling processes. MMP7 is expressed in multiple tissues including the glandular epithelia of the breast prostate and lung.

Biological function summary

MMP7 participates in wound healing embryonic development and tissue invasion processes. It does not typically form large complexes but can interact with other ECM components and inhibitors like TIMPs (tissue inhibitors of metalloproteinases). MMP7 has specific roles in regulating cellular environments by modifying the ECM and influencing the bioavailability of growth factors.

Pathways

MMP7 plays significant roles in pathways like the Wnt and MAPK signaling pathways. In the Wnt pathway MMP7 modulates the availability of bound matrix proteins influencing signaling dynamics. It interacts with proteins such as B-catenin and influences downstream gene expression. In the context of MAPK pathways MMP7 activity can lead to signal transduction changes that impact cell proliferation and migration.

Associated diseases and disorders

MMP7 has associations with cancer progression and fibrosis. In colorectal cancer for example MMP7 expression levels correlate with tumor invasion and metastasis. It interacts with proteins like E-cadherin which affects cell adhesion properties and promotes disease advancement. In pulmonary fibrosis excessive MMP7 activity contributes to matrix degradation and aberrant tissue repair highlighting its relevance in pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on Human gastric carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/2000 dilution

    Lane 1: A549 (Human lung carcinoma) whole cell lysate at 20 µg

    Lane 2: BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution

    Lane 1: Human colon cancer lysate at 10 µg

    Lane 2: Human tonsil lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution

    All lanes: Human prostate lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Immunohistochemical analysis of paraffin-embedded Human prostatic carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on Human prostatic carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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