JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154507

Anti-MMP8 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal MMP8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MMP8 aa 50 to C-terminus.

View Alternative Names

CLG1, MMP8, Neutrophil collagenase, Matrix metalloproteinase-8, PMNL collagenase, MMP-8, PMNL-CL

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MMP8 antibody (AB154507)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MMP8 antibody (AB154507)

Immunofluorescent analysis of methanol-fixed A431 cells labeling MMP8 with ab154507 at 1/500 dilution. Image at the bottom costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody (AB154507)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody (AB154507)

Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling MMP8 with ab154507 antibody at 1/500.

Western blot - Anti-MMP8 antibody (AB154507)
  • WB

Unknown

Western blot - Anti-MMP8 antibody (AB154507)

7.5% SDS PAGE

All lanes:

Western blot - Anti-MMP8 antibody (ab154507) at 1/10000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MMP8 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P22894

style
left-column

Alternative Products

Primary Antibodies

AB53017

Anti-MMP8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MMP8 antibody (AB53017)

  • 4
  • 7
Primary Antibodies

AB211967

PE Anti-MMP8 antibody [EP1252Y]

RabMAb

Flow Cytometry (Intracellular) - PE Anti-MMP8 antibody [EP1252Y] (AB211967)

  • 0
  • 0
Primary Antibodies

AB224265

Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free

RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

  • 0
  • 0
Primary Antibodies

AB81286

Anti-MMP8 antibody [EP1252Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

  • 4
  • 5

Complementary Products

Assay Kits

AB219050

Human MMP8 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MMP8 ELISA Kit (AB219050)

  • 0
  • 0
Primary Antibodies

AB243107

Anti-ILT-4 antibody [EPR22081] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107)

  • 0
  • 0
Primary Antibodies

AB52631

Anti-MMP1 antibody [EP1247Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631)

  • 5
  • 5
Primary Antibodies

AB52915

Anti-MMP3 antibody [EP1186Y]

KO Validated|RabMAb|Recombinant

Western blot - Anti-MMP3 antibody [EP1186Y] (AB52915)

  • 5
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.
Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Can degrade fibrillar type I, II, and III collagens.
See full target information MMP8
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:15 PubMed39901302

2025

Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Senhui Wei,Ying Li,Jing Zhou,Yongming Xia

Molecular medicine reports 25: PubMed35169867

2022

Inhibitory effect of membrane‑free stem cell components derived from adipose tissues on skin inflammation in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Eun Ha,Preethi Vetrivel,Seong Min Kim,Pritam Bhagwan Bhosale,Hun Hwan Kim,Jung Eun Pak,Jeong Doo Heo,Young Sil Kim,Gon Sup Kim
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com