AB81286

Anti-MMP8 antibody [EP1252Y]

Name: Anti-MMP8 antibody [EP1252Y] (ab81286) | Abcam
Brand: Abcam Limited
SKU: AB81286
Price: 320 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(47 Publications)

Rabbit Recombinant Monoclonal MMP8 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 47 publications.

View Alternative Names

CLG1, MMP8, Neutrophil collagenase, Matrix metalloproteinase-8, PMNL collagenase, MMP-8, PMNL-CL

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (AB81286)

Purified ab81286 at 1/50 dilution (2μg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input) : Rat lung lysate 10μg
Lane 2 (+) : ab81286 + Rat lung lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 70 kDa

All lanes:

Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

Dilution and blocking buffer : 5% NFDM/TBST

Negative control : mouse brain.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

Dilution and blocking buffer : 5% NFDM/TBST

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

All lanes:

Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution

All lanes:

Rat lung lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 72 kDa

false

578 PE

PE Anti-MMP8 antibody [EP1252Y]
Carrier free

Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1252Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.

Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Can degrade fibrillar type I, II, and III collagens.

See full target information MMP8

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Publications (47)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:2073-2086 PubMed39990284

2025

Synergistic Enhancement of Therapeutic Efficacy in Acute Myocardial Infarction via Nanoflower-Like MnO Nanozymes in Coordination with Adipose-Derived Stem Cell Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Gao,Demeng Xia,Xiaoyong Zhang,Yao Xiao,Hong Zhou,Gan Chen,Haibin Wang

Scientific reports 14:31086 PubMed39730651

2024

A novel biomarker of COVI-19: MMP8 emerged by integrated bulk RNAseq and single-cell sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Liu,Shunda Wang

International journal of molecular sciences 25: PubMed39273553

2024

Investigating the Anti-Inflammatory, Analgesic, and Chondroprotective Effects of (Thunb.) Makino in Osteoarthritis: An In Vitro and In Vivo Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hee-Geun Jo,Chae Yun Baek,Yeseul Hwang,Eunhye Baek,Chanyoon Park,Ho Sueb Song,Donghun Lee

Heliyon 10:e32494 PubMed38948030

2024

Integrating network pharmacology and experimental evaluation to explore the complementary therapeutic effect and mechanism of melatonin in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kamoran Tuerhong,Kehao Liu,Danfeng Shen,Qianyu Zhang,Qi Huang,Mingcong Yang,Ziyu Huang,Lu Wang,Sheng Yang,Yuzhou Li

International journal of biological sciences 19:2848-2859 PubMed37324952

2023

Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Wanfu Wu,Mozhgan Sarhadi,Xiaoyu Song,Jingling Xue,Yubing Dai,Jan-Ake Gustafsson

International journal of molecular sciences 24: PubMed37240089

2023

Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Tomaszewska,Halyna Rudyk,Siemowit Muszyński,Monika Hułas-Stasiak,Norbert Leszczyński,Maria Mielnik-Błaszczak,Janine Donaldson,Piotr Dobrowolski

Journal of pineal research 75:e12872 PubMed37057370

2023

Melatonin inhibits chondrosarcoma cell proliferation and metastasis by enhancing miR-520f-3p production and suppressing MMP7 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Tran Nguyen,Chih-Yang Lin,Ting-Kuo Chang,Yi-Chin Fong,Louis Anoop Thadevoos,Chao-Yang Lai,Yuan-Li Huang,Chun-Hao Tsai,Chih-Yuan Ko,Ju-Fang Liu,Shun-Fa Yang,Chih-Hsin Tang

Journal of clinical and translational hepatology 11:1035-1049 PubMed37577240

2023

Therapeutic Effect of Prolyl Endopeptidase Inhibitor in High-fat Diet-induced Metabolic Dysfunction-associated Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Bin Zhang,Meng-Ting Li,Shuang-Zhe Lin,Yu-Qing Cheng,Jian-Gao Fan,Yuan-Wen Chen

Cancers 14: PubMed35884594

2022

LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Jiang,Xiangpan Li,Yu Yang,Jiajun Luo,Xunshan Ren,Jingwen Yuan,Qiang Tong

Acta dermato-venereologica 102:adv00749 PubMed35604238

2022

Biomarkers of Skin Graft Healing in Venous Leg Ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus Kirketerp-Møller,Petra Doerfler,Nicole Schoefmann,Barbara Wolff-Winiski,Omid Niazi,Vibeke Pless,Tonny Karlsmark,Magnus S. Ågren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP8 antibody [EP1252Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (AB81286)

Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (AB81286)

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (47)

Product promise