Rabbit Recombinant Monoclonal MMP8 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 39 publications.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
IHC-P | IP | WB | |
---|---|---|---|
Human | Tested | Expected | Expected |
Mouse | Not recommended | Expected | Tested |
Rat | Expected | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Can degrade fibrillar type I, II, and III collagens.
CLG1, MMP8, Neutrophil collagenase, Matrix metalloproteinase-8, PMNL collagenase, MMP-8, PMNL-CL
Rabbit Recombinant Monoclonal MMP8 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 39 publications.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.
MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.
MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.
MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Purified ab81286 at 1/50 dilution (2μg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10μg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa
All lanes: Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)
Predicted band size: 53 kDa
All lanes: Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution
All lanes: Rat lung lysate at 10 µg
All lanes: HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 72 kDa
ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dilution and blocking buffer: 5% NFDM/TBST
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution
All lanes: Mouse lung tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70 kDa
Exposure time: 180s
Dilution and blocking buffer: 5% NFDM/TBST
Negative control: mouse brain.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70 kDa
Exposure time: 10s
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