Skip to main content

Rabbit Recombinant Monoclonal MMP8 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Expected
Expected
Mouse
Not recommended
Expected
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Can degrade fibrillar type I, II, and III collagens.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal MMP8 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1252Y

Purification technique

Affinity purification Protein A

Specificity

WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab224265 is the carrier-free version of ab81286.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Activity summary

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

Associated diseases and disorders

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265), expandable thumbnail

    Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    Purified ab81286 at 1/50 dilution (2μg) immunoprecipitating MMP8 in Rat lung lysate.
    Lane 1 (input): Rat lung lysate 10μg
    Lane 2 (+): ab81286 + Rat lung lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 70 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

    All lanes: Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (AB81286)

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    This IHC data was generated using the same anti-MMP8 antibody clone [EP1252Y] in a different buffer formulation (cat# ab81286).

    ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265), expandable thumbnail

    Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

    Dilution and blocking buffer: 5% NFDM/TBST

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286) at 1/1000 dilution

    All lanes: Mouse lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 70 kDa

    Exposure time: 180s

    Dilution and blocking buffer: 5% NFDM/TBST

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

  • Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265), expandable thumbnail

    Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

    Dilution and blocking buffer: 5% NFDM/TBST

    Negative control: mouse brain.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MMP8 antibody [EP1252Y] (AB81286) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 70 kDa

    Exposure time: 10s

    Dilution and blocking buffer: 5% NFDM/TBST

    Negative control: mouse brain.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com