Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

This IHC data was generated using the same anti-MMP8 antibody clone [EP1252Y] in a different buffer formulation (cat# ab81286).

ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IP

Unknown

Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

Purified ab81286 at 1/50 dilution (2μg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input) : Rat lung lysate 10μg
Lane 2 (+) : ab81286 + Rat lung lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 70 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

All lanes:

Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (<a href='/en-us/products/primary-antibodies/mmp8-antibody-ep1252y-ab81286'>ab81286</a>)

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

Dilution and blocking buffer : 5% NFDM/TBST

Negative control : mouse brain.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP8 antibody [EP1252Y] (<a href='/en-us/products/primary-antibodies/mmp8-antibody-ep1252y-ab81286'>ab81286</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (AB224265)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

Dilution and blocking buffer : 5% NFDM/TBST

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP8 antibody [EP1252Y] (<a href='/en-us/products/primary-antibodies/mmp8-antibody-ep1252y-ab81286'>ab81286</a>) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s