AB58803

Anti-MMP9 antibody [56-2A4]

Name: Anti-MMP9 antibody [56-2A4] (ab58803) | Abcam
Brand: Abcam Limited
SKU: AB58803
Price: 770 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(133 Publications)

Anti-MMP9 antibody [56-2A4] (ab58803) is a mouse monoclonal antibody detecting MMP9 in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Guinea pig, Human, Mouse, Rabbit, Rat.

- Over 110 publications
- Trusted since 2007

View Alternative Names

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

1 Images

Supplier Data

Western blot - Anti-MMP9 antibody [56-2A4] (AB58803)

1 : 500 dilution of ab58803 detected MMP-9 on 10 μg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9

All lanes:

Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)

Predicted band size: 78 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

56-2A4

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human, Rabbit, Guinea pig, Rat, Mouse

Applications

ICC/IF, IHC-Fr, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MMP9 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P14780

Specificity

Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9.Does not react with MMP1, MMP2, MMP3 or MMP13.The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-MMP9 antibody [56-2A4] (ab58803) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Guinea pig, Human, Mouse, Rabbit, Rat samples.

What is the molecular weight of MMP9?
Anti-MMP9 [56-2A4] (ab58803) specifically detects a band for MMP9 (UniProt: P14780) at a molecular weight of 78kDa.

Trusted by the scientific community
Anti-MMP9 [56-2A4] (ab58803) was first used in a scientific publication in 2007 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-MMP9 [56-2A4] (ab58803) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Is purified.

Storage buffer

pH: 7 Preservative: 0.1% Sodium azide Constituents: 1.64% Sodium phosphate, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).

Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene MMP9 is a matrix metalloproteinase that plays an essential role in the local proteolysis of the extracellular matrix and in leukocyte migration. It cleaves KiSS1 at a Gly-|-Leu bond and processes NINJ1 to generate the Secreted ninjurin-1 form. Additionally, MMP9 cleaves type IV and type V collagen into large C-terminal three-quarter fragments and shorter N-terminal one-quarter fragments. It degrades fibronectin but not laminin or Pz-peptide. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MMP9

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Publications (133)

Recent publications for all applications. Explore the full list and refine your search

Pakistan journal of pharmaceutical sciences 38:1632-1646 PubMed40996179

2025

Cantharidic acid inhibits the malignant progression of colorectal cancer by inhibiting aerobic glycolysis and regulating the PI3K/Akt/P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wei,Shulin Dai,Dongyun Zhang,Ting Zhang,Xiaoyu Wang,Bolin Liu,Wei Huang,Yin Li,Mingliao Niu

Parasitology research 124:97 PubMed40890376

2025

Restorative potential of melatonin during treatment of severe scabies in an experimental model.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud S Sharaf,Ahmad A Othman,Dareen M Ali,Abdallah M Hafez,Asmaa R Ellakany

Communications biology 8:1230 PubMed40817179

2025

Targeting VCAM-1 with chimeric antigen receptor and regulatory T cell for abdominal aortic aneurysm treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwei Gang,Yu Lun,Xiaoxu Zhang,Jamol Uzokov,Han Jiang,Yuchen He,Shikai Shen,Shiyue Wang,Philipp Erhart,Dittmar Böckler,Yuemeng Li,Yanshuo Han,Jian Zhang

Cancer cell international 25:49 PubMed39962568

2025

Comprehensive network pharmacology and experimental study to investigate the effect and mechanism of solasonine on breast carcinoma treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkai Ge,Min Gao,Yingqi Dai,Gang Zheng,Li Yang,Wenshu Zuo,Xingsong Tian

Scandinavian journal of immunology 100:e13420 PubMed39511761

2024

Serum integrin accumulation during asthma exacerbation: The role of matrix metalloproteinases.

Applications

Unspecified application

Species

Unspecified reactive species

Tellez-Jimenez Olivia,Trejo-Jasso Christian,Ramos-Ramirez Patricia,Chapela Rocío,Miguel-Reyes José Luis,López-Estrada Erika Del Carmen,Bazán-Perkins Blanca

Scientific reports 14:21806 PubMed39300144

2024

Dexamethasone acetate loaded poly(ε-caprolactone) nanofibers for rat corneal chemical burn treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Da Ran Kim,Sun-Kyoung Park,Eun Jeong Kim,Dong-Kyu Kim,Young Chae Yoon,David Myung,Hyun Jong Lee,Kyung-Sun Na

Biology of reproduction 110:971-984 PubMed38335245

2024

Membrane inflammasome activation by choriodecidual Ureaplasma parvum infection without intra-amniotic infection in a Non-Human Primate model†.

Applications

Unspecified application

Species

Unspecified reactive species

Sudeshna Tripathy,Irina Burd,Meredith A Kelleher

Frontiers in pharmacology 14:1288406 PubMed38293673

2024

Integrating RNA-sequencing and network analysis to explore the mechanism of topical Pien Tze Huang treatment on diabetic wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Zhao Cao,Liang-Liang Tian,Jing-Yi Hou,Yi Zhang,He Xu,Hong-Jun Yang,Jing-Jing Zhang

The Journal of pharmacology and experimental therapeutics 388:469-483 PubMed37316330

2024

Establishing a Dexamethasone Treatment Regimen To Alleviate Sulfur Mustard-Induced Corneal Injuries in a Rabbit Model.

Applications

Unspecified application

Species

Unspecified reactive species

Neha Mishra,Rama Kant,Kushal Kandhari,Neera Tewari-Singh,Poojya Anantharam,Claire R Croutch,Mina B Pantcheva,J Mark Petrash,Houmam Araj,Chapla Agarwal,Rajesh Agarwal

Medicine 102:e35157 PubMed37747031

2023

Molecular basis of breast cancer with comorbid depression and the mechanistic insights of Xiaoyaosan in treating breast cancer-associated depression.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP9 antibody [56-2A4]

Western blot - Anti-MMP9 antibody [56-2A4] (AB58803)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (133)

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