Anti-MMP9 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody (AB73734)

IHC image of MMP9 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73734, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Project

Western blot - Anti-MMP9 antibody (AB73734)

The 95 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to MMP9.

All lanes:

Western blot - Anti-MMP9 antibody (ab73734) at 1 µg/mL

All lanes:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 78 kDa

Observed band size: 40 kDa,95 kDa

false

Exposure time: 12min

• WB

Unknown

Western blot - Anti-MMP9 antibody (AB73734)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab73734 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406,

All lanes:

Western blot - Anti-MMP9 antibody (ab73734) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (<a href='/en-us/products/proteins-peptides/recombinant-human-mmp9-protein-proenzyme-ab82955'>ab82955</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Anti-MMP9 antibody (AB73734)

All lanes:

Western blot - Anti-MMP9 antibody (ab73734) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (<a href='/en-us/products/proteins-peptides/recombinant-human-mmp9-protein-proenzyme-ab82955'>ab82955</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

true

Exposure time: 2min