AB188156

Anti-MMS19 antibody

Name: Anti-MMS19 antibody (ab188156) | Abcam
Brand: Abcam Limited
SKU: AB188156

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(2 Publications)

Rabbit Polyclonal MMS19 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MMS19 aa 650-800.

View Alternative Names

MMS19L, MMS19, MMS19 nucleotide excision repair protein homolog, hMMS19, MET18 homolog, MMS19-like protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

Caco-2 cells stained for MMS19 (green) using ab188156 at 2 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

Paraffin embedded human testis tissue stained for MMS19 using ab188156 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MMS19 antibody (AB188156)

All lanes:

Western blot - Anti-MMS19 antibody (ab188156) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG sp cell lysate

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MMS19 aa 650-800. The exact immunogen used to generate this antibody is proprietary information.

Q96T76

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MMS19 also known as MMS19L or mitotic spindle assembly 19 plays an important role in DNA repair and ribonucleotide reduction. It is a component of the cytoplasmic iron-sulfur protein assembly (CIA) machinery. MMS19 is approximately 104 kDa in mass and is expressed in a variety of tissues with higher expression noted in testis and thymus. The protein has been observed in the nucleus and cytoplasm where it participates in DNA-related and metabolic processes.

Biological function summary

MMS19 functions as a core component of the cytoplasmic iron-sulfur protein assembly (CIA) targeting complex. This complex targets specific iron-sulfur cluster-containing proteins which are essential for DNA repair and other cellular processes. MMS19 facilitates the incorporation of iron-sulfur clusters into target proteins which is critical for their function. It interacts with other proteins like CIAO1 and FAM96B within the complex indicating its collaborative role in cellular maintenance and genomic stability.

Pathways

MMS19 is actively engaged in the DNA repair and replication pathways. It plays a role in nucleotide excision repair (NER) and is important in maintaining genomic integrity through its involvement in DNA synthesis and repair. The protein associates with helicase-like transcription factor (HLTF) and other related proteins within these pathways helping coordinate the response to DNA damage and repair mechanisms.

MMS19's dysfunction has been linked to various cancer types due to its central role in DNA repair. Defects in MMS19 can lead to genomic instability a hallmark of cancerous transformations. Moreover its interaction with other proteins in the repair pathways such as ERCC2 links it to disorders like xeroderma pigmentosum. This connection suggests its importance in protecting against DNA damage and maintaining genomic stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity (PubMed : 29848660). In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed : 22678361, PubMed : 22678362, PubMed : 23585563, PubMed : 29225034). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD (PubMed : 20797633). Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis (PubMed : 29848660). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed : 11279242).

See full target information MMS19

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 292:13879-13889 PubMed28615450

2017

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.

Applications

Unspecified application

Species

Unspecified reactive species

Arunkumar S Upadhyay,Oliver Stehling,Christakis Panayiotou,Ralf Rösser,Roland Lill,Anna K Överby

DNA repair 43:9-17 PubMed27235625

2016

Human DNA polymerase ε is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana N Moiseeva,Armin M Gamper,Brian L Hood,Thomas P Conrads,Christopher J Bakkenist

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MMS19 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

Western blot - Anti-MMS19 antibody (AB188156)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise