Rabbit Polyclonal MMS19 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MMS19 aa 900-1000 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MMS19 aa 900-1000 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T76
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity (PubMed:29848660). In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678361, PubMed:22678362, PubMed:23585563, PubMed:29225034). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD (PubMed:20797633). Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis (PubMed:29848660). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).
Alternative names
MMS19L, MMS19, MMS19 nucleotide excision repair protein homolog, hMMS19, MET18 homolog, MMS19-like protein
Recommended products
Rabbit Polyclonal MMS19 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MMS19 aa 900-1000 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MMS19 aa 900-1000 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T76
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MMS19 also known as MMS19L or mitotic spindle assembly 19 plays an important role in DNA repair and ribonucleotide reduction. It is a component of the cytoplasmic iron-sulfur protein assembly (CIA) machinery. MMS19 is approximately 104 kDa in mass and is expressed in a variety of tissues with higher expression noted in testis and thymus. The protein has been observed in the nucleus and cytoplasm where it participates in DNA-related and metabolic processes.
- Biological function summary
MMS19 functions as a core component of the cytoplasmic iron-sulfur protein assembly (CIA) targeting complex. This complex targets specific iron-sulfur cluster-containing proteins which are essential for DNA repair and other cellular processes. MMS19 facilitates the incorporation of iron-sulfur clusters into target proteins which is critical for their function. It interacts with other proteins like CIAO1 and FAM96B within the complex indicating its collaborative role in cellular maintenance and genomic stability.
- Pathways
MMS19 is actively engaged in the DNA repair and replication pathways. It plays a role in nucleotide excision repair (NER) and is important in maintaining genomic integrity through its involvement in DNA synthesis and repair. The protein associates with helicase-like transcription factor (HLTF) and other related proteins within these pathways helping coordinate the response to DNA damage and repair mechanisms.
- Associated diseases and disorders
MMS19's dysfunction has been linked to various cancer types due to its central role in DNA repair. Defects in MMS19 can lead to genomic instability a hallmark of cancerous transformations. Moreover its interaction with other proteins in the repair pathways such as ERCC2 links it to disorders like xeroderma pigmentosum. This connection suggests its importance in protecting against DNA damage and maintaining genomic stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (ab214328)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling MMS19 with ab214328 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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