Rabbit Polyclonal MMS2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2V2 aa 1 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human UBE2V2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15819
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Alternative names
Ubiquitin-conjugating enzyme E2 variant 2, DDVit 1, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, MMS2 homolog, Vitamin D3-inducible protein, EDAF-1, EDPF-1, UEV2, MMS2, UBE2V2
Recommended products
Rabbit Polyclonal MMS2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBE2V2 aa 1 to C-terminus.
Alternative names
Ubiquitin-conjugating enzyme E2 variant 2, DDVit 1, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, MMS2 homolog, Vitamin D3-inducible protein, EDAF-1, EDPF-1, UEV2, MMS2, UBE2V2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human UBE2V2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15819
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Keep as concentrated solution.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMS2 protein also known as UBE2V2 is a ubiquitin-conjugating enzyme variant found within cells. Unlike typical ubiquitin-conjugating enzymes MMS2 lacks the conserved active site cysteine. The MMS2 protein has a molecular mass of approximately 16 kDa and is expressed in a range of tissues including those of the human immune system. Functionally MMS2 partners with another enzyme Ubc13 to facilitate the assembly of Lys63-linked polyubiquitin chains necessary for DNA damage tolerance and repair processes.
- Biological function summary
MMS2 plays a critical role in cellular responses to DNA damage. It is part of the Ubc13-MMS2 complex which contributes to the post-replication repair pathway. This complex modulates the signaling and resolution of DNA double-strand breaks. MMS2 through its role in this complex impacts cellular mechanisms for maintaining genomic integrity and preventing genomic instability.
- Pathways
MMS2 takes part in the DNA damage response and NF-kappaB signaling pathways. In the process of DNA repair it works with proteins like Rad6 and Rad18 enabling cells to overcome stalled replication forks and promoting cell survival in response to DNA damage. In the NF-kappaB pathway MMS2 contributes to signaling cascades that activate immune responses and inflammation working alongside other proteins to mediate cellular responses.
- Associated diseases and disorders
MMS2 is linked to cancer and neurodegenerative diseases. Its role in DNA repair and signaling pathways connects it to tumor suppression with defects in MMS2 function potentially leading to increased susceptibility to cancer due to accumulated DNA damage. Additionally MMS2's interaction with proteins involved in neuroinflammation suggests a possible connection to neurodegenerative conditions such as Alzheimer’s disease where dysregulated inflammation and repair pathways contribute to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labelling MMS2 with ab155007 at 1/500 dilution.
Western blot - Anti-MMS2 antibody (ab155007)
15% SDS PAGE
All lanes: Western blot - Anti-MMS2 antibody (ab155007) at 1/1000 dilution
All lanes: BCL-1 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Western blot - Anti-MMS2 antibody (ab155007)
15% SDS PAGE
All lanes: Western blot - Anti-MMS2 antibody (ab155007) at 1/1000 dilution
All lanes: HL-60 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS2 antibody (ab155007)
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, labelling MMS2 with ab155007 at 1/500 dilution.
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