Rabbit Recombinant Monoclonal MMS22L (C6ORF167) antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--immunoprecipitation-img171884.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)")
![Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--western-blot-img179829.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)")
![Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--flow-cytometry-intracellular-img164529.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)")
Publications
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- Nucleic acids research 51:4760-47732023Symmetric control of sister chromatid cohesion establishment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxin Zhang,Lili Li,Yu Miao,Xiaojing Liu,Haitao Sun,Meiqian Jiang,Xiaoli Li,Zhen Li,Cong Liu,Baohua Liu,Xingzhi Xu,Qinhong Cao,Wenya Hou,Chunlai Chen,Huiqiang LouPubMed 36912084
- Frontiers in genetics 13:10259702022Integrative pan-cancer landscape of MMS22L and its potential role in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiting Guo,Fahui Liu,Qiming GongPubMed 36276962
- Frontiers in oncology 11:7116422021MMS22L Expression as a Predictive Biomarker for the Efficacy of Neoadjuvant Chemoradiotherapy in Oesophageal Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiyu Luo,Wenwu He,Tianqin Mao,Xuefeng Leng,Hong Wu,Wen Li,Xuyang Deng,Tingci Zhao,Ming Shi,Chuan Xu,Yongtao HanPubMed 34660277
- PLoS genetics 15:e10076852019Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitao Sun et. al.PubMed 30779731
- Molecular cell 69:879-892.e52018The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Hsiang Huang et. al.PubMed 29478807
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes - |
Species Rat | Dilution info 1/20 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/20 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target data
Function
Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:21113133, PubMed:26527279, PubMed:27338793, PubMed:29478807). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:27797818). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA): the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:29478807). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed:27797818, PubMed:29478807). Within the complex, MMS22L acts by binding ssDNA (PubMed:27797818).
Alternative names
C6orf167, MMS22L, Protein MMS22-like, Methyl methanesulfonate-sensitivity protein 22-like
Recommended products
Rabbit Recombinant Monoclonal MMS22L (C6ORF167) antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13654
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMS22L (C6ORF167) is a protein that plays a significant role in DNA damage response and repair. It is also known as a part of a protein complex that ensures genomic stability. MMS22L has a mass of approximately 51 kDa and is found expressed in various tissues notably in those with proliferative capacity such as testis and thymus. This protein becomes active upon DNA replication stress and enhances the stabilization of replication forks.
- Biological function summary
MMS22L associates with several other proteins to form the MMS22L-TONSL complex which is critical for repairing DNA double-strand breaks. This complex facilitates homologous recombination repair by assisting in the recruitment of important repair factors. Through its functionality MMS22L plays a critical part in maintaining genomic stability ensuring that cells replicate accurately and efficiently.
- Pathways
MMS22L integrates into the homologous recombination repair pathway and the Fanconi anemia pathway. In its activities it collaborates with proteins like TONSL and RAD51 which are central in maintaining the integrity of DNA through accurate repair. These pathways highlight MMS22L’s involvement in repairing DNA damages particularly during the S and G2 phases of the cell cycle.
- Associated diseases and disorders
MMS22L has connections to conditions like cancer and Fanconi anemia where genomic instability is a common feature. Dysregulation or mutations affecting MMS22L can impede DNA repair mechanisms thereby contributing to the development of these disorders. In cancer MMS22L's role connects with proteins like BRCA2 highlighting its importance in pathways that when disrupted lead to tumor development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--immunoprecipitation-img171884.jpg "Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
Purified ab181047 at 1:20 dilution (0.5μg) immunoprecipitating MMS22L (C6ORF167) in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg.
Lane 2 (+): ab181047 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181047 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDaAll lanes: Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
Predicted band size: 142 kDa
![Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--western-blot-img179829.jpg "Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
All lanes: Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3: LLC1 (Mouse lung carcinoma ) whole cell lysate
Lane 4: Mouse testis lysate
Lane 5: Mouse spleen lysate
Lane 6: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 142 kDa
![Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047), expandable thumbnail](https://content.abcam.com/products/images/mms22l-c6orf167-antibody-epr13654-ab181047--flow-cytometry-intracellular-img164529.jpg "Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MMS22L (C6ORF167) with Purified ab181047 at 1:20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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