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AB181047

Anti-MMS22L (C6ORF167) antibody [EPR13654]

4

(2 Reviews)

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(5 Publications)

Rabbit Recombinant Monoclonal MMS22L (C6ORF167) antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

C6orf167, MMS22L, Protein MMS22-like, Methyl methanesulfonate-sensitivity protein 22-like

3 Images
Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MMS22L (C6ORF167) with Purified ab181047 at 1 : 20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)
  • IP

Lab

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)

Purified ab181047 at 1 : 20 dilution (0.5µg) immunoprecipitating MMS22L (C6ORF167) in HeLa whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/20 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab181047 + HeLa whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab181047 in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 142 kDa

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Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)
  • WB

Unknown

Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)

All lanes:

Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

LLC1 (Mouse lung carcinoma ) whole cell lysate at 20 µg

Lane 4:

Mouse testis lysate at 20 µg

Lane 5:

Mouse spleen lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 142 kDa

false

  • Carrier free

    Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13654

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

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ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMS22L (C6ORF167) is a protein that plays a significant role in DNA damage response and repair. It is also known as a part of a protein complex that ensures genomic stability. MMS22L has a mass of approximately 51 kDa and is found expressed in various tissues notably in those with proliferative capacity such as testis and thymus. This protein becomes active upon DNA replication stress and enhances the stabilization of replication forks.
Biological function summary

MMS22L associates with several other proteins to form the MMS22L-TONSL complex which is critical for repairing DNA double-strand breaks. This complex facilitates homologous recombination repair by assisting in the recruitment of important repair factors. Through its functionality MMS22L plays a critical part in maintaining genomic stability ensuring that cells replicate accurately and efficiently.

Pathways

MMS22L integrates into the homologous recombination repair pathway and the Fanconi anemia pathway. In its activities it collaborates with proteins like TONSL and RAD51 which are central in maintaining the integrity of DNA through accurate repair. These pathways highlight MMS22L’s involvement in repairing DNA damages particularly during the S and G2 phases of the cell cycle.

MMS22L has connections to conditions like cancer and Fanconi anemia where genomic instability is a common feature. Dysregulation or mutations affecting MMS22L can impede DNA repair mechanisms thereby contributing to the development of these disorders. In cancer MMS22L's role connects with proteins like BRCA2 highlighting its importance in pathways that when disrupted lead to tumor development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 21113133, PubMed : 26527279, PubMed : 27338793, PubMed : 29478807). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 27797818). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA) : the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 29478807). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed : 27797818, PubMed : 29478807). Within the complex, MMS22L acts by binding ssDNA (PubMed : 27797818).
See full target information MMS22L
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:4760-4773 PubMed36912084

2023

Symmetric control of sister chromatid cohesion establishment.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Zhang,Lili Li,Yu Miao,Xiaojing Liu,Haitao Sun,Meiqian Jiang,Xiaoli Li,Zhen Li,Cong Liu,Baohua Liu,Xingzhi Xu,Qinhong Cao,Wenya Hou,Chunlai Chen,Huiqiang Lou

Frontiers in genetics 13:1025970 PubMed36276962

2022

Integrative pan-cancer landscape of MMS22L and its potential role in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiting Guo,Fahui Liu,Qiming Gong

Frontiers in oncology 11:711642 PubMed34660277

2021

MMS22L Expression as a Predictive Biomarker for the Efficacy of Neoadjuvant Chemoradiotherapy in Oesophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyu Luo,Wenwu He,Tianqin Mao,Xuefeng Leng,Hong Wu,Wen Li,Xuyang Deng,Tingci Zhao,Ming Shi,Chuan Xu,Yongtao Han

PLoS genetics 15:e1007685 PubMed30779731

2019

Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Sun,Jiaxin Zhang,Siyu Xin,Meiqian Jiang,Jingjing Zhang,Zhen Li,Qinhong Cao,Huiqiang Lou

Molecular cell 69:879-892.e5 PubMed29478807

2018

The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Hsiang Huang,Faith Fowler,Chin-Chuan Chen,Zih-Jie Shen,Barry Sleckman,Jessica K Tyler
View all publications
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Product promise

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