AB250307

Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MMS22L (C6ORF167) antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

3 Images

Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

This data was developed using ab250307, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MMS22L (C6ORF167) with Purified ab250307 at 1 : 20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Lab

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181047).
Purified ab181047 at 1 : 20 dilution (0.5µg) immunoprecipitating MMS22L (C6ORF167) in HeLa whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (<a href='/en-us/products/primary-antibodies/mms22l-c6orf167-antibody-epr13654-ab181047'>ab181047</a>) at 1/20 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/mms22l-c6orf167-antibody-epr13654-ab181047'>ab181047</a> + HeLa whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/mms22l-c6orf167-antibody-epr13654-ab181047'>ab181047</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 142 kDa

false

Unknown

Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

All lanes:

Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (<a href='/en-us/products/primary-antibodies/mms22l-c6orf167-antibody-epr13654-ab181047'>ab181047</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

LLC1 (Mouse lung carcinoma ) whole cell lysate at 20 µg

Lane 4:

Mouse testis lysate at 20 µg

Lane 5:

Mouse spleen lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 142 kDa

false

Unconjugated

Anti-MMS22L (C6ORF167) antibody [EPR13654]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13654

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250307 is the carrier-free version of ab181047.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMS22L (C6ORF167) is a protein that plays a significant role in DNA damage response and repair. It is also known as a part of a protein complex that ensures genomic stability. MMS22L has a mass of approximately 51 kDa and is found expressed in various tissues notably in those with proliferative capacity such as testis and thymus. This protein becomes active upon DNA replication stress and enhances the stabilization of replication forks.

Biological function summary

MMS22L associates with several other proteins to form the MMS22L-TONSL complex which is critical for repairing DNA double-strand breaks. This complex facilitates homologous recombination repair by assisting in the recruitment of important repair factors. Through its functionality MMS22L plays a critical part in maintaining genomic stability ensuring that cells replicate accurately and efficiently.

Pathways

MMS22L integrates into the homologous recombination repair pathway and the Fanconi anemia pathway. In its activities it collaborates with proteins like TONSL and RAD51 which are central in maintaining the integrity of DNA through accurate repair. These pathways highlight MMS22L’s involvement in repairing DNA damages particularly during the S and G2 phases of the cell cycle.

MMS22L has connections to conditions like cancer and Fanconi anemia where genomic instability is a common feature. Dysregulation or mutations affecting MMS22L can impede DNA repair mechanisms thereby contributing to the development of these disorders. In cancer MMS22L's role connects with proteins like BRCA2 highlighting its importance in pathways that when disrupted lead to tumor development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 21113133, PubMed : 26527279, PubMed : 27338793, PubMed : 29478807). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 27797818). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA) : the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 29478807). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed : 27797818, PubMed : 29478807). Within the complex, MMS22L acts by binding ssDNA (PubMed : 27797818).

See full target information MMS22L

Alternative Names

C6orf167, MMS22L, Protein MMS22-like, Methyl methanesulfonate-sensitivity protein 22-like

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] - BSA and Azide free (AB250307)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise