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AB169546

Anti-MNAT1 antibody [EPR10940]

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(2 Publications)

Rabbit Recombinant Monoclonal MNAT1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CAP35, MAT1, RNF66, MNAT1, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, Cyclin-G1-interacting protein, Menage a trois, RING finger protein 66, RING finger protein MAT1, p35, p36

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR10940] (AB169546)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR10940] (AB169546)

Immunofluorescent analysis of HepG2 cells labeling MNAT1 with ab169546 at 1/100 dilution.

Western blot - Anti-MNAT1 antibody [EPR10940] (AB169546)
  • WB

Unknown

Western blot - Anti-MNAT1 antibody [EPR10940] (AB169546)

All lanes:

Western blot - Anti-MNAT1 antibody [EPR10940] (ab169546) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from HeLa cell lysate

Predicted band size: 36 kDa

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Western blot - Anti-MNAT1 antibody [EPR10940] (AB169546)
  • WB

Unknown

Western blot - Anti-MNAT1 antibody [EPR10940] (AB169546)

All lanes:

Western blot - Anti-MNAT1 antibody [EPR10940] (ab169546) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10940

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MNAT1 also known as menage à trois 1 functions as a component of the CDK-activating kinase complex. It weighs about 32 kilodaltons. MNAT1 plays a role in regulating cell cycle progression. It expresses widely in various tissues but shows higher levels in proliferating tissues due to its involvement in cell division. Its expression pattern suggests a significant function in maintaining proper cell cycle control.
Biological function summary

This protein serves as a regulatory subunit of the CDK7-cyclin H-MNAT1 complex. This complex forms the core of CDK-activating kinase which is essential for the activation of other cyclin-dependent kinases (CDKs). By doing so MNAT1 influences transcription regulation and DNA repair processes. As part of this complex MNAT1 modulates the activity of the proteins involved in a variety of cellular mechanisms. Through these activities MNAT1 helps in maintaining genomic integrity and proper cellular response to DNA damage.

Pathways

The activity of MNAT1 is involved in the cell cycle regulation pathway and the transcription regulation process. It plays a role in phosphorylating other CDKs directly influencing their activation and consequently cell cycle control. MNAT1 associates closely with proteins such as cyclin H and CDK7 within these pathways. This collaboration ensures that the essential checkpoints in the cell cycle are correctly regulated and that transcription processes are executed properly.

Irregularities in MNAT1 function can correlate with cancer development due to its central role in cell cycle regulation and genomic stability. Altered expression levels or mutations in MNAT1 can disrupt normal cell division leading to unchecked cell proliferation. Furthermore MNAT1 interacts with cyclin H and CDK7 which are also important in the proper regulation of cell cycle checkpoints and can also influence cancer pathways when dysfunctional. Hence MNAT1 is a potential target for therapeutic strategies aiming to control abnormal cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
See full target information MNAT1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed40627336

2025

Use of adipose-derived stem cells on decellularized bladder scaffolds for functional bladder mucosa regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Cesar Ulises Monjaras-Avila,Ana Cecilia Luque-Badillo,Nicholas J Carr,Anthony A Papp,Alan I So,Claudia Chavez-Munoz

The Journal of biological chemistry 298:102433 PubMed36041630

2022

Systematic mutagenesis of TFIIH subunit p52/Tfb2 identifies residues required for XPB/Ssl2 subunit function and genetic interactions with TFB6.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob Bassett,Jenna K Rimel,Shrabani Basu,Pratik Basnet,Jie Luo,Krysta L Engel,Michael Nagel,Alexander Woyciehowsky,Christopher C Ebmeier,Craig D Kaplan,Dylan J Taatjes,Jeffrey A Ranish
View all publications
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