AB154802

Anti-MNAT1 antibody [EPR2555(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MNAT1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CAP35, MAT1, RNF66, MNAT1, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, Cyclin-G1-interacting protein, Menage a trois, RING finger protein 66, RING finger protein MAT1, p35, p36

3 Images

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Intracellular flow cytometric analysis of permeabilized Hela cells labeling MAT1 with ab154802 at 1/10 dilution (red) or a negative rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Immunofluorescence analysis of HeLa cells labeling MAT1 with ab154802 at 1/100 dilution.

Unknown

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

All lanes:

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (ab154802) at 1/1000 dilution

Lane 1:

A431 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

A673 lysate at 10 µg

Lane 4:

RAW264.7 lysate at 10 µg

Predicted band size: 36 kDa

false

Carrier free

Anti-MNAT1 antibody [EPR2555(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2555(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MNAT1 also known as menage à trois 1 functions as a component of the CDK-activating kinase complex. It weighs about 32 kilodaltons. MNAT1 plays a role in regulating cell cycle progression. It expresses widely in various tissues but shows higher levels in proliferating tissues due to its involvement in cell division. Its expression pattern suggests a significant function in maintaining proper cell cycle control.

Biological function summary

This protein serves as a regulatory subunit of the CDK7-cyclin H-MNAT1 complex. This complex forms the core of CDK-activating kinase which is essential for the activation of other cyclin-dependent kinases (CDKs). By doing so MNAT1 influences transcription regulation and DNA repair processes. As part of this complex MNAT1 modulates the activity of the proteins involved in a variety of cellular mechanisms. Through these activities MNAT1 helps in maintaining genomic integrity and proper cellular response to DNA damage.

Pathways

The activity of MNAT1 is involved in the cell cycle regulation pathway and the transcription regulation process. It plays a role in phosphorylating other CDKs directly influencing their activation and consequently cell cycle control. MNAT1 associates closely with proteins such as cyclin H and CDK7 within these pathways. This collaboration ensures that the essential checkpoints in the cell cycle are correctly regulated and that transcription processes are executed properly.

Irregularities in MNAT1 function can correlate with cancer development due to its central role in cell cycle regulation and genomic stability. Altered expression levels or mutations in MNAT1 can disrupt normal cell division leading to unchecked cell proliferation. Furthermore MNAT1 interacts with cyclin H and CDK7 which are also important in the proper regulation of cell cycle checkpoints and can also influence cancer pathways when dysfunctional. Hence MNAT1 is a potential target for therapeutic strategies aiming to control abnormal cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

See full target information MNAT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 29:1111-1124 PubMed29496966

2018

C3G dynamically associates with nuclear speckles and regulates mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Dhruv Kumar Shakyawar,Bhattiprolu Muralikrishna,Vegesna Radha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MNAT1 antibody [EPR2555(2)]

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise