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AB316211

Anti-MNDA antibody [253A] - BSA and Azide free

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Mouse Recombinant Monoclonal MNDA antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MNDA.

View Alternative Names

Myeloid cell nuclear differentiation antigen, MNDA

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)

This data was developed using ab188566, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte cell) cells labelling MNDA with ab188566 at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in THP-1 cell line (shown in green). Negative control : HeLa.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counter stain tubulin at a 1/500 dilution (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)

This data was developed using ab188566, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MNDA with ab188566 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.
he section was incubated with ab188566 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)

This data was developed using ab188566, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MNDA with ab188566 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The section was incubated with ab188566 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)
  • WB

Lab

Western blot - Anti-MNDA antibody [253A] - BSA and Azide free (AB316211)

This data was developed using ab188566, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) was used as a loading control at a 1/100000 dilution.

Negative control : HeLa (PMID : 10492038).

All lanes:

Western blot - Anti-MNDA antibody [253A] (<a href='/en-us/products/primary-antibodies/mnda-antibody-253a-ab188566'>ab188566</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 47 kDa

false

Exposure time: 37s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

253A

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MNDA.

P41218

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Reactivity data

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Product details

ab316211 is the carrirer-free version of ab188566.

Product Specifications
Anti-MNDA antibody [253A] (ab188566) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-MNDA antibody [253A] (ab188566) specifically detects MNDA (UniProt ID: P41218; Molecular weight: 45kDa) and is sold in 100µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-MNDA antibody [253A] (ab188566) has high sensitivity and specificity.

This product has switched from a hybridoma to recombinant production method on 20th June 2025

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MNDA known as Myeloid Cell Nuclear Differentiation Antigen has a mass of approximately 45 kDa. Expression occurs mainly in myeloid cells including monocytes and granulocytes. It is encoded by the MNDA gene situated on chromosome 1q22. MNDA belongs to the HIN-200 family of proteins which are involved in various cellular activities. The protein contains a DNA-binding domain suggesting its role in transcriptional regulation.
Biological function summary

The function of MNDA is critical for the immune response and cellular differentiation. MNDA participates in regulating gene transcription in response to external stimuli. It operates in complex with other nuclear proteins to modulate the immune and inflammatory response aiming for a precise cellular behavior. MNDA's presence in myeloid cells signifies its role in the innate immune system contributing significantly to cell maturation and defense mechanisms.

Pathways

MNDA influences gene expression modifications tied to key immune response pathways. It has a noted involvement in the Toll-like receptor (TLR) signaling pathway where it helps mediate responses to pathogens. MNDA interacts with proteins such as TLR4 which plays a vital role in innate immunity. Furthermore MNDA participates in the interferon regulatory factor (IRF) pathway impacting type I interferon production and immune regulation.

MNDA has associations with myeloid malignancies and autoimmune conditions. Abnormal expression or mutation of MNDA might contribute to disease pathology in acute myeloid leukemia (AML) where it may function alongside proteins like RUNX1 a transcription factor important in hematopoiesis. Additionally MNDA expressions and mutations link to systemic lupus erythematosus (SLE) where its regulatory impact on the immune response may be disrupted influencing inflammatory pathways and immune system regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
See full target information MNDA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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