Anti-MNK1 antibody [RP23040203]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MNK1 Antibody [RP23040203] (AB313481)

Immunofluorescent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling MNK1 with ab313481 at 2 ?g/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of MNK1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of MNK1. Panel e)represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-MNK1 Antibody [RP23040203] (AB313481)

All lanes:

Western blot - Anti-MNK1 Antibody [RP23040203] (ab313481) at 1 µg/mL

Lane 1:

MCF7 cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Lane 3:

HEK293 cell lysate at 30 µg

Lane 4:

Neuro-2A cell lysate at 30 µg

Lane 5:

C2C12 cell lysate at 30 µg

Lane 6:

HeLa cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 51 kDa

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