Anti-MNK1 (phospho T385) antibody [EPR2370]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNK1 (phospho T385) antibody [EPR2370] (AB109102)

Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] (AB109102)

All lanes:

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate, treated with Calyculin A. at 10 µg

Predicted band size: 51 kDa

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• WB

CiteAb

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] (AB109102)

MNK1 (phospho T385) western blot using anti-MNK1 (phospho T385) antibody [EPR2370] ab109102. Publication image and figure legend from Zhao, Q., Zhu, H. P., et al., 2020, Int J Mol Sci, PubMed 32156008.

ab109102 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109102 please see the product overview.

(A) Cells were incubated with various concentrations (0, 20, or 40 μg/mL) of DHP1808 for 24 h. Expression or phosphorylation levels of HSP90 client proteins were determined by western blot analysis; (B) Cells were incubated with various concentrations (0, 20, or 40 μg/mL) of DHP1808 for 24 h. Expression or phosphorylation levels of proteins associated with MAPK signaling pathway were determined by western blot analysis; (C) Cells were incubated with various concentrations (0, 20, or 40 μg/mL) of DHP1808 for 24 h. Expression or phosphorylation level of proteins associated with the PI3K-Akt signaling pathway was determined by western blot analysis; (D). The fold change of EGFR expression was determined by qPCR analysis.

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