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AB247758

Anti-MNK1 (phospho T385) antibody [EPR2370] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MNK1 phospho T385 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MNK1, MKNK1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, MAPK signal-integrating kinase 1, Mnk1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNK1 (phospho T385) antibody [EPR2370] - BSA and Azide free (AB247758)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNK1 (phospho T385) antibody [EPR2370] - BSA and Azide free (AB247758)

This data was developed using ab109102, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] - BSA and Azide free (AB247758)
  • WB

Unknown

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] - BSA and Azide free (AB247758)

This data was developed using ab109102, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MNK1 (phospho T385) antibody [EPR2370] (<a href='/en-us/products/primary-antibodies/mnk1-phospho-t385-antibody-epr2370-ab109102'>ab109102</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate, treated with Calyculin A. at 10 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2370

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247758 is the carrier-free version of ab109102.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitogen-activated protein kinase-interacting serine/threonine-protein kinase 1 (MNK1) also known as MAP kinase-interacting kinase 1 is an enzyme with a molecular weight of approximately 51 kDa. MNK1 plays an important role in the regulation of protein synthesis by phosphorylating eukaryotic initiation factor 4E (eIF4E). Researchers find high expression of MNK1 in many tissues especially within the brain and immune system which suggests its involvement in diverse cellular processes.
Biological function summary

The protein kinase MNK1 regulates the translation of mRNA by forming part of the signaling complex that includes eIF4E. This complex plays a role in stress response cell growth and the immune response. Protein synthesis regulated by MNK1 affects cellular activities by selectively translating specific mRNAs related to those processes. MNK1's specific regulation often depends on signals received through upstream kinases.

Pathways

MNK1 is a part of the mitogen-activated protein kinase (MAPK) signaling cascade particularly the extracellular signal-regulated kinase (ERK) and p38 MAPK pathways. These pathways find involvement in transmitting signals from the cell membrane to the nucleus affecting gene expression and cellular responses. MNK1 modulates its activity in response to signals from upstream proteins such as ERK1/2 or p38α/β which are likely to influence the regulation of eIF4E and therefore translation initiation.

Scientists observe MNK1 implications in cancer progression and neurodegenerative diseases. MNK1-mediated phosphorylation of proteins like eIF4E and its overactivation often correlates with tumor growth and resistance to chemotherapy. Additionally research links MNK1's altered activity to neurodegenerative conditions such as Alzheimer's disease. Interactions between MNK1 and other proteins like eIF4E suggest potential roles for MNK1 in these disease pathologies making it an attractive target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
See full target information MKNK1 pT385
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com