Rabbit Polyclonal MNT antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MNT aa 1-50.
View Alternative Names
BHLHD3, ROX, MNT, Max-binding protein MNT, Class D basic helix-loop-helix protein 3, Myc antagonist MNT, Protein ROX, bHLHd3
- WB
Unknown
Western blot - Anti-mnt antibody (AB129466)
All lanes:
Western blot - Anti-mnt antibody (ab129466) at 0.1 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
293T whole cell lysate at 50 µg
Lane 4:
Jurkat whole cell lysate at 50 µg
Predicted band size: 62 kDa
false
- IP
Unknown
Immunoprecipitation - Anti-mnt antibody (AB129466)
Detection of mnt by Western Blot of Immunoprecipitates.
ab129466 at 1μg/ml staining mnt in HeLa whole cell lysate immunoprecipitated using ab129466 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-mnt antibody (ab129466)
Predicted band size: 62 kDa
false
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Mnt functions to regulate cell proliferation and differentiation by acting as a negative regulator. Mnt often forms a complex with Max to repress the transcription of Myc target genes. Myc proteins promote cell cycle progression and growth while Mnt exerts an opposing effect by working as a transcriptional repressor. This delicate balance between Myc activation and Mnt repression is necessary to maintain controlled cell proliferation.
Pathways
Mnt regulates essential cellular processes through the Myc/Max/Mad network and the E2F transcription factor pathway. Within the Myc/Max/Mad network mnt balances out the activity of Myc by forming a competing dimer with Max. In its interaction with the E2F pathway mnt indirectly influences cell cycle progression. These pathways include numerous proteins like Mad1 and Mad3 which also interact with Mnt to modulate transcription.
Product protocols
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Target data
Product promise
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