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AB317194

Anti-MNT antibody [HL2309]

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Rabbit Monoclonal MNT antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MNT.

View Alternative Names

BHLHD3, ROX, MNT, Max-binding protein MNT, Class D basic helix-loop-helix protein 3, Myc antagonist MNT, Protein ROX, bHLHd3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MNT antibody [HL2309] (AB317194)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MNT antibody [HL2309] (AB317194)

ab317194 detects MNT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : MNT stained by ab317194 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNT antibody [HL2309] (AB317194)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNT antibody [HL2309] (AB317194)

ab317194 detects MNT protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse testis. MNT stained by ab317194 diluted at 1 : 200. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-MNT antibody [HL2309] (AB317194)
  • WB

Supplier Data

Western blot - Anti-MNT antibody [HL2309] (AB317194)

A431 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317194 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-MNT antibody [HL2309] (ab317194) at 1/2000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

A431 nuclear extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-MNT antibody [HL2309] (AB317194)
  • WB

Supplier Data

Western blot - Anti-MNT antibody [HL2309] (AB317194)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317194 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-MNT antibody [HL2309] (ab317194) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2309

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MNT.

Q99583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'mnt' protein known as Max's Next Tango is a transcriptional repressor with a significant role in gene expression regulation. Mnt has a molecular mass of approximately 34 kDa and belongs to the basic helix-loop-helix (bHLH) family. It forms homodimers or heterodimers with Max another member of the bHLH family. Mnt is expressed in several tissues and cell types including liver brain and muscle tissue. Its presence in diverse tissues indicates a multifaceted role in basic cellular function.
Biological function summary

Mnt functions to regulate cell proliferation and differentiation by acting as a negative regulator. Mnt often forms a complex with Max to repress the transcription of Myc target genes. Myc proteins promote cell cycle progression and growth while Mnt exerts an opposing effect by working as a transcriptional repressor. This delicate balance between Myc activation and Mnt repression is necessary to maintain controlled cell proliferation.

Pathways

Mnt regulates essential cellular processes through the Myc/Max/Mad network and the E2F transcription factor pathway. Within the Myc/Max/Mad network mnt balances out the activity of Myc by forming a competing dimer with Max. In its interaction with the E2F pathway mnt indirectly influences cell cycle progression. These pathways include numerous proteins like Mad1 and Mad3 which also interact with Mnt to modulate transcription.

Mnt has a recognized connection with cancer and neurodegenerative diseases. Abnormal expression or mutations in mnt can disrupt the balance of cell proliferation often leading to tumorigenesis. In neurodegenerative diseases improper regulation of Myc and Mnt affects neurogenesis and neuronal survival. Proteins like p53 often implicated in these diseases show interconnected pathways with Mnt highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
See full target information MNT
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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