Rabbit Polyclonal MOB1A antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MOB1A.
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- Communications biology 5:7802022Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRossella Delle Donne,Rosa Iannucci,Laura Rinaldi,Luca Roberto,Maria A Oliva,Emanuela Senatore,Domenica Borzacchiello,Luca Lignitto,Giorgio Giurato,Francesca Rizzo,Assunta Sellitto,Francesco Chiuso,Salvatore Castaldo,Giovanni Scala,Virginia Campani,Valeria Nele,Giuseppe De Rosa,Chiara D'Ambrosio,Corrado Garbi,Andrea Scaloni,Alessandro Weisz,Concetta Ambrosino,Antonella Arcella,Antonio FelicielloPubMed 35918402
- Journal of ovarian research 15:232022Up-regulation of MTHFD2 is associated with clinicopathological characteristics and poor survival in ovarian cancer, possibly by regulating MOB1A signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangrong Cui,Huancheng Su,Jiaolin Yang,Xueqing Wu,Kai Huo,Xuan Jing,Sanyuan ZhangPubMed 35135596
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MOB1A. Database link Q9H8S9
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
Target data
Function
Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.
Alternative names
C2orf6, MOB4B, MOBK1B, MOBKL1B, MOB1A, MOB kinase activator 1A, Mob1 alpha, Mob1 homolog 1B, Mps one binder kinase activator-like 1B, Mob1A
Recommended products
Rabbit Polyclonal MOB1A antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MOB1A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MOB1A. Database link Q9H8S9
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mob1A also known as Mps One Binder kinase activator-like 1A is a protein involved in cell signaling pathways. It has a molecular mass of approximately 25 kDa. Mob1A is found in various tissues but it shows elevated expression levels in the liver kidney and lung. Its structural attributes contribute to interacting with other cellular proteins assisting in regulation of cell cycle progression.
- Biological function summary
Mob1A acts as a core component of the Hippo signaling pathway playing an important role in cell proliferation and apoptosis regulation. It forms a complex with large tumor suppressor kinase 1 (LATS1) and facilitates interaction with other pathway components. The interaction with LATS1 enhances the phosphorylation activity which then has further consequences for cellular processes. This function is critical for preventing uncontrolled cell growth and ensuring proper organ size is maintained.
- Pathways
Mob1A participates in the Hippo and Wnt signaling pathways. Within the Hippo pathway Mob1A works closely with proteins such as YAP/TAZ which are regulatory proteins important for gene expression control. In the Wnt pathway Mob1A contributes indirectly to cell fate determination. These connections highlight its importance in managing cellular growth and differentiation requiring careful orchestration of its interactions.
- Associated diseases and disorders
Mob1A shows significant involvement in cancer and liver diseases. Dysregulation within the Hippo pathway where Mob1A plays an important role often leads to tumorigenesis due to increased cellular proliferation. Research shows correlations between altered Mob1A function and hepatocellular carcinoma where Mob1A interacts with other proteins like MST1/2 to influence disease progression. Understanding these interactions may offer therapeutic insights for managing such conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Western blot - Anti-Mob1A antibody (ab236969)
All lanes: Western blot - Anti-Mob1A antibody (ab236969) at 1/200 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2: Mouse small intestine tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (ab236969)
Paraffin-embedded human small intestine tissue stained for Mob1A using ab236969 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Mob1A antibody (ab236969)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mob1A (Green) using ab236969 at 1/333 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Immunoprecipitation - Anti-Mob1A antibody (ab236969)
Mob1A was immunoprecipitated from 500 μg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 1: Rabbit control IgG instead of ab236969 in Jurkat cell lysate.
Lane 2: ab236969 IP in Jurkat cell lysate.
Lane 3: Jurkat whole cell lysate (input).
All lanes: Immunoprecipitation - Anti-Mob1A antibody (ab236969)
Predicted band size: 25 kDa
Western blot - Anti-Mob1A antibody (ab236969)
All lanes: Western blot - Anti-Mob1A antibody (ab236969) at 1/200 dilution
All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (ab236969)
Paraffin-embedded human breast cancer tissue stained for Mob1A using ab236969 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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