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AB236969

Anti-Mob1A antibody

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(4 Publications)

Rabbit Polyclonal MOB1A antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MOB1A.

View Alternative Names

C2orf6, MOB4B, MOBK1B, MOBKL1B, MOB1A, MOB kinase activator 1A, Mob1 alpha, Mob1 homolog 1B, Mps one binder kinase activator-like 1B, Mob1A

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (AB236969)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (AB236969)

Paraffin-embedded human small intestine tissue stained for Mob1A using ab236969 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Mob1A antibody (AB236969)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mob1A antibody (AB236969)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mob1A (Green) using ab236969 at 1/333 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (AB236969)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (AB236969)

Paraffin-embedded human breast cancer tissue stained for Mob1A using ab236969 at 1/1000 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitation - Anti-Mob1A antibody (AB236969)
  • IP

Supplier Data

Immunoprecipitation - Anti-Mob1A antibody (AB236969)

Mob1A was immunoprecipitated from 500 μg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).

Lane 1 : Rabbit control IgG instead of ab236969 in Jurkat cell lysate.

Lane 2 : ab236969 IP in Jurkat cell lysate.

Lane 3 : Jurkat whole cell lysate (input).

All lanes:

Immunoprecipitation - Anti-Mob1A antibody (ab236969)

Predicted band size: 25 kDa

false

Western blot - Anti-Mob1A antibody (AB236969)
  • WB

Supplier Data

Western blot - Anti-Mob1A antibody (AB236969)

All lanes:

Western blot - Anti-Mob1A antibody (ab236969) at 1/200 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Mouse small intestine tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 25 kDa

false

Western blot - Anti-Mob1A antibody (AB236969)
  • WB

Supplier Data

Western blot - Anti-Mob1A antibody (AB236969)

All lanes:

Western blot - Anti-Mob1A antibody (ab236969) at 1/200 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MOB1A.

Q9H8S9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mob1A also known as Mps One Binder kinase activator-like 1A is a protein involved in cell signaling pathways. It has a molecular mass of approximately 25 kDa. Mob1A is found in various tissues but it shows elevated expression levels in the liver kidney and lung. Its structural attributes contribute to interacting with other cellular proteins assisting in regulation of cell cycle progression.
Biological function summary

Mob1A acts as a core component of the Hippo signaling pathway playing an important role in cell proliferation and apoptosis regulation. It forms a complex with large tumor suppressor kinase 1 (LATS1) and facilitates interaction with other pathway components. The interaction with LATS1 enhances the phosphorylation activity which then has further consequences for cellular processes. This function is critical for preventing uncontrolled cell growth and ensuring proper organ size is maintained.

Pathways

Mob1A participates in the Hippo and Wnt signaling pathways. Within the Hippo pathway Mob1A works closely with proteins such as YAP/TAZ which are regulatory proteins important for gene expression control. In the Wnt pathway Mob1A contributes indirectly to cell fate determination. These connections highlight its importance in managing cellular growth and differentiation requiring careful orchestration of its interactions.

Mob1A shows significant involvement in cancer and liver diseases. Dysregulation within the Hippo pathway where Mob1A plays an important role often leads to tumorigenesis due to increased cellular proliferation. Research shows correlations between altered Mob1A function and hepatocellular carcinoma where Mob1A interacts with other proteins like MST1/2 to influence disease progression. Understanding these interactions may offer therapeutic insights for managing such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.
See full target information MOB1A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 3:100740 PubMed36099918

2022

A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Olav M Andersen,Nikolaj Bøgh,Anne M Landau,Gro G Pløen,Anne Mette G Jensen,Giulia Monti,Benedicte P Ulhøi,Jens R Nyengaard,Kirsten R Jacobsen,Margarita M Jørgensen,Ida E Holm,Marianne L Kristensen,Aage Kristian O Alstrup,Esben S S Hansen,Charlotte E Teunissen,Laura Breidenbach,Mathias Droescher,Ying Liu,Hanne S Pedersen,Henrik Callesen,Yonglun Luo,Lars Bolund,David J Brooks,Christoffer Laustsen,Scott A Small,Lars F Mikkelsen,Charlotte B Sørensen

Communications biology 5:780 PubMed35918402

2022

Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rossella Delle Donne,Rosa Iannucci,Laura Rinaldi,Luca Roberto,Maria A Oliva,Emanuela Senatore,Domenica Borzacchiello,Luca Lignitto,Giorgio Giurato,Francesca Rizzo,Assunta Sellitto,Francesco Chiuso,Salvatore Castaldo,Giovanni Scala,Virginia Campani,Valeria Nele,Giuseppe De Rosa,Chiara D'Ambrosio,Corrado Garbi,Andrea Scaloni,Alessandro Weisz,Concetta Ambrosino,Antonella Arcella,Antonio Feliciello

Journal of ovarian research 15:23 PubMed35135596

2022

Up-regulation of MTHFD2 is associated with clinicopathological characteristics and poor survival in ovarian cancer, possibly by regulating MOB1A signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrong Cui,Huancheng Su,Jiaolin Yang,Xueqing Wu,Kai Huo,Xuan Jing,Sanyuan Zhang

Cancer management and research 13:4829-4840 PubMed34168502

2021

Downregulation of MicroRNA-130a Inhibits Oral Squamous Cell Carcinoma Proliferation and Metastasis via the Hippo-YAP Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiran Peng,Shoushan Hu,Kun Zhang,Yuru Wang,Maierdanjiang Rouzi,Dan Zhou,Ran Yang
View all publications
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