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AB151015

Anti-MOCOS/MCS antibody

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(1 Publication)

Rabbit Polyclonal MOCOS/MCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Molybdenum cofactor sulfurase, MCS, MOS, MoCo sulfurase, hMCS, Molybdenum cofactor sulfurtransferase, MOCOS

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MOCOS/MCS antibody (AB151015)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MOCOS/MCS antibody (AB151015)

RT4 cells stained for MOCOS/MCS (green) using ab151015 at 2 μg/ml dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCOS/MCS antibody (AB151015)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCOS/MCS antibody (AB151015)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labelling MOCOS/MCS with ab151015 at 1/50 dilution

Western blot - Anti-MOCOS/MCS antibody (AB151015)
  • WB

Supplier Data

Western blot - Anti-MOCOS/MCS antibody (AB151015)

All lanes:

Western blot - Anti-MOCOS/MCS antibody (ab151015)

Lane 1:

Molecular ladder

Lane 2:

RT4 cell lysate at 0.4 µg/mL

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

The antibody solution should be gently mixed before use.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Molybdenum cofactor sulfurase (MOCOS) also known as MCS is an important enzyme in sulfur transfer for the activation of molybdenum cofactor. MOCOS weighs approximately 88 kDa and is found in a variety of tissues including liver and kidney. It plays a critical role in the biochemical process of activating the molybdenum cofactor which is essential for the proper function of certain enzymes.
Biological function summary

MOCOS contributes significantly to a sulfurase complex that includes other enzymes responsible for molybdenum cofactor biosynthesis and activation. It specifically introduces sulfur into the cofactor which is important for the functionality of molybdenum-dependent enzymes like xanthine dehydrogenase and aldehyde oxidase. MOCOS's activity impacts not just individual enzyme functions but whole biochemical pathways governed by these enzymes.

Pathways

MOCOS's biological function integrates into the purine degradation pathway and the aldehyde detoxification pathway. The enzyme works collaboratively with xanthine dehydrogenase in purine metabolism enabling the conversion of hypoxanthine to xanthine and subsequently to uric acid. In the aldehyde detoxification pathway MOCOS-associated enzymes such as aldehyde oxidase contribute to the breakdown of aromatic aldehydes and heterocyclic compounds playing a role in metabolizing pharmaceuticals and toxins.

MOCOS deficiencies relate to xanthinuria Type II characterized by an excessive accumulation of xanthine due to impaired xanthine dehydrogenase activity. Individuals with dysfunctional MOCOS can experience xanthine oxidation defects leading to potential renal complications due to stone formation. This relates closely to aldehyde oxidase whose reduced activity might impact drug metabolism and increase susceptibility to certain aldehyde-induced cellular damages.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sulfurates the molybdenum cofactor (PubMed : 34356852). Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form (PubMed : 34356852). In vitro, the C-terminal domain is able to reduce N-hydroxylated prodrugs, such as benzamidoxime (PubMed : 16973608).
See full target information MOCOS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 291:18514-24 PubMed27402836

2016

Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan Baumann,Thierry Hennet
View all publications
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Product promise

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