AB229827

Anti-MOCS3 antibody

Name: Anti-MOCS3 antibody (ab229827) | Abcam
Brand: Abcam Limited
SKU: AB229827

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(1 Publication)

Rabbit Polyclonal MOCS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus.

View Alternative Names

UBA4, MOCS3, Adenylyltransferase and sulfurtransferase MOCS3, Molybdenum cofactor synthesis protein 3, Molybdopterin synthase sulfurylase, MPT synthase sulfurylase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (AB229827)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (AB229827)

Paraffin-embedded human brain tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (AB229827)

Paraffin-embedded human liver tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MOCS3 antibody (AB229827)

All lanes:

Western blot - Anti-MOCS3 antibody (ab229827) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95396

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MOCS3 also known as Molybdenum Cofactor Synthesis 3 is an important enzyme involved in the biosynthesis of the molybdenum cofactor. It has a molecular mass of approximately 101 kDa. This enzyme localizes mainly in the cytoplasm and plays a role as a partner in the transfer of sulfur in the form of a thiocarboxylate to L-cysteine. MOCS3 helps catalyze the synthesis of the MPT precursor Z ligand which is important for the formation of active molybdenum cofactor a molecule required for various enzymatic reactions in the body.

Biological function summary

MOCS3 is integral to the tRNA thiolation and molybdenum cofactor biosynthesis processes. It participates in the formation of molybdopterin an organophosphate essential for the activity of the molybdenum enzymes. MOCS3 forms a complex with MOCS2A and MOCS2B collaborating to activate these enzymes. This association highlights its importance in ensuring the synthesis of vital cofactors that impact enzyme function.

Pathways

MOCS3 functionsally integrates into the molybdenum cofactor biosynthesis pathway and the tRNA thiolation pathway. These pathways ensure the correct function of molybdoenzymes and the proper configuration of tRNA molecules. MOCS3 interacts with the MOCS1 protein in the molybdenum cofactor pathway where they cooperatively synthesize components essential for the activity of molybdoenzymes. This connection ensures the proper formation and function of cellular enzymes that rely on molybdenum cofactor.

MOCS3 dysfunction can lead to molybdenum cofactor deficiency a rare but serious disorder that disrupts the body’s ability to metabolize sulfite leading to neurological damage. It connects with the GPHN protein in this context as both are involved in maintaining correct molybdoenzyme function. Additionally abnormalities in MOCS3 have been linked to intellectual disability showcasing its significance in maintaining proper neurological health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 acting as a sulfur donor for thiocarboxylation reactions.

See full target information MOCS3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:911715 PubMed35795047

2022

CircASPH Promotes Hepatocellular Carcinoma Progression Through Methylation and Expression of HAO2.

Applications

Unspecified application

Species

Unspecified reactive species

Han Zhuo,Jinguo Xia,Jin Zhang,Junwei Tang,Sheng Han,Qitong Zheng,Deming Zhu,Feihong Zhang,Zhenggang Xu,Dongwei Sun,Zhongming Tan,Chen Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MOCS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (AB229827)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (AB229827)

Western blot - Anti-MOCS3 antibody (AB229827)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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