Rabbit Polyclonal MOCS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95396
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 acting as a sulfur donor for thiocarboxylation reactions.
Alternative names
UBA4, MOCS3, Adenylyltransferase and sulfurtransferase MOCS3, Molybdenum cofactor synthesis protein 3, Molybdopterin synthase sulfurylase, MPT synthase sulfurylase
Recommended products
Rabbit Polyclonal MOCS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MOCS3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95396
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MOCS3 also known as Molybdenum Cofactor Synthesis 3 is an important enzyme involved in the biosynthesis of the molybdenum cofactor. It has a molecular mass of approximately 101 kDa. This enzyme localizes mainly in the cytoplasm and plays a role as a partner in the transfer of sulfur in the form of a thiocarboxylate to L-cysteine. MOCS3 helps catalyze the synthesis of the MPT precursor Z ligand which is important for the formation of active molybdenum cofactor a molecule required for various enzymatic reactions in the body.
- Biological function summary
MOCS3 is integral to the tRNA thiolation and molybdenum cofactor biosynthesis processes. It participates in the formation of molybdopterin an organophosphate essential for the activity of the molybdenum enzymes. MOCS3 forms a complex with MOCS2A and MOCS2B collaborating to activate these enzymes. This association highlights its importance in ensuring the synthesis of vital cofactors that impact enzyme function.
- Pathways
MOCS3 functionsally integrates into the molybdenum cofactor biosynthesis pathway and the tRNA thiolation pathway. These pathways ensure the correct function of molybdoenzymes and the proper configuration of tRNA molecules. MOCS3 interacts with the MOCS1 protein in the molybdenum cofactor pathway where they cooperatively synthesize components essential for the activity of molybdoenzymes. This connection ensures the proper formation and function of cellular enzymes that rely on molybdenum cofactor.
- Associated diseases and disorders
MOCS3 dysfunction can lead to molybdenum cofactor deficiency a rare but serious disorder that disrupts the body’s ability to metabolize sulfite leading to neurological damage. It connects with the GPHN protein in this context as both are involved in maintaining correct molybdoenzyme function. Additionally abnormalities in MOCS3 have been linked to intellectual disability showcasing its significance in maintaining proper neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-MOCS3 antibody (ab229827)
All lanes: Western blot - Anti-MOCS3 antibody (ab229827) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (ab229827)
Paraffin-embedded human liver tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS3 antibody (ab229827)
Paraffin-embedded human brain tissue stained for MOCS3 using ab229827 at 1/100 dilution in immunohistochemical analysis.
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