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AB282241

Anti-Moesin antibody [EPR2428-7] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Moesin antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

Moesin, Membrane-organizing extension spike protein, MSN

1 Images
Sandwich ELISA - Anti-Moesin antibody [EPR2428-7] - BSA and Azide free (Detector) (AB282241)
  • sELISA

Lab

Sandwich ELISA - Anti-Moesin antibody [EPR2428-7] - BSA and Azide free (Detector) (AB282241)

Sandwich ELISA of ab253768 with the capture antibody ab282239 dilution at 2 µg/mL and detector antibody ab282241 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2428-7

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab282241 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat Moesin. The recommended pair for sandwich ELISA is:
Capture: ab282239, Human/Mouse/Rat Moesin Capture Antibody (unconjugated)
Detector: ab282241, Human/Mouse/Rat Moesin Detector Antibody (unconjugated)
The reference range value is 1.56 - 100 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Moesin also known as MSN or moesin protein is part of the ERM (ezrin radixin moesin) family which links the actin cytoskeleton to the plasma membrane. It weighs around 68 kDa. This protein is widely expressed in various tissues notably in endothelial cells and other cells with high cytoskeletal dynamics. Moesin plays an essential role in maintaining cell shape polarity and enabling cellular processes like migration and adhesion.
Biological function summary

This protein serves as a crosslinker between the plasma membrane and the actin cytoskeleton influencing signal transduction pathways. Moesin participates significantly in cellular processes like cytokinesis and microvilli formation by binding actin filaments. It is part of several functional complexes ensuring proper cytoskeletal organization and cellular dynamics. Moesin's interactions with other proteins like Rho GTPases regulate its functions aiding cellular morphology and polarity.

Pathways

Moesin is actively involved in the RhoA-ROCK pathway. This pathway is fundamental to actin cytoskeletal reorganization and cell contraction. Moesin interacts with proteins such as radixin and ezrin ensuring cohesion in cytoskeletal rearrangements. Through these interactions Moesin contributes to cell motility and various signaling cascades necessary for cellular responses.

Moesin is associated with conditions such as cancer and immune system disorders. Aberrant expression of moesin can contribute to enhanced cellular migration and invasion which are mechanisms seen in cancer metastasis. Moesin's interaction with proteins like ezrin in cancer progression has been documented. Also in immune disorders altered moesin activity can affect leukocyte transmigration disrupting efficient immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton (PubMed : 10212266). Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement (PubMed : 10212266). These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction (PubMed : 12387735, PubMed : 15039356). The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs (PubMed : 9298994, PubMed : 9616160). Modulates phagolysosomal biogenesis in macrophages (By similarity). Participates also in immunologic synapse formation (PubMed : 27405666).
See full target information Moesin
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Product promise

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