Anti-Moesin antibody [MSN/492] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular carcinoma tissue labeling Moesin with ab215840 at 1 μg/ml dilution.

• ICC

Supplier Data

Immunocytochemistry - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

This data was developed using ab216033, the same antibody clone in a different buffer formulation.

Immunocytochemistry analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Moesin with ab216033 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

• WB

Supplier Data

Western blot - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

This data was developed using ab216033, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Moesin antibody [MSN/492] - BSA and Azide free (ab215840)

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 68 kDa

false

• WB

Lab

Western blot - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

False colour image of Western blot : Anti-Moesin antibody [MSN/492] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab216033 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Moesin antibody [MSN/492] (<a href='/en-us/products/primary-antibodies/moesin-antibody-msn-492-ab216033'>ab216033</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

• WB

Supplier Data

Western blot - Anti-Moesin antibody [MSN/492] - BSA and Azide free (AB215840)

This data was developed using ab216033, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Moesin antibody [MSN/492] - BSA and Azide free (ab215840)

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Predicted band size: 68 kDa

false