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AB317242

Anti-Monkeypox virus A4L antibody [HL2555]

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Rabbit Monoclonal antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Monkeypox virus, Monkeypox virus, Transfected cell line - Monkeypox virus samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)

ab317242 detects Monkeypox virus A4L protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : Monkeypox virus A4L stained by ab317242 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)
  • WB

Supplier Data

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)

Non-infected (–) and infected (+) BSC-40 whole cell extracts (5 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317242 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (ab317242) at 1/1000 dilution

Lane 1:

Non-infected (–) BSC-40 whole cell extracts at 5 µg

Lane 2:

Vaccinia virus infected (+) BSC-40 whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)
  • WB

Supplier Data

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (AB317242)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317242 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Monkeypox virus A4L antibody [HL2555] (ab317242) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged Monkeypox virus A4L transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2555

Isotype

IgG

Carrier free

No

Reacts with

Monkeypox virus

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Monkeypox virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Monkeypox virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Monkeypox virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monkeypox virus A4L also known as DNA polymerase catalytic subunit plays a critical role in viral replication. This protein with an approximate mass of 110 kDa is expressed in the cytoplasm of infected host cells. A4L functions mechanistically as a DNA-dependent DNA polymerase facilitating the elongation of the DNA strand during replication. It achieves this by catalyzing the addition of deoxynucleotides to the 3'-end of the growing DNA chain using the viral DNA as a template.
Biological function summary

A4L is pivotal for the propagation of the monkeypox virus ensuring successful duplication of the viral genome. As an essential component of the viral replication complex it interacts with other viral and host proteins to execute its function. Its activity is indispensable for the replication cycle which directly influences the virus's ability to infect new cells and propagate throughout the host organism.

Pathways

Monkeypox virus A4L integrates into the virus replication and repair pathways. It interacts with replication proteins such as E9L DNA polymerase orchestrating a multifaceted machinery critical for viral DNA synthesis. A4L's role also aligns with recombination pathways where it participates in repairing damaged viral DNA ensuring the integrity and continuity of viral replication.

The activity of A4L is closely associated with the development and progression of monkeypox infection in humans. The protein's function is central to the pathogenicity of the virus implicating it in skin lesions and systemic involvement characteristic of the disease. The presence of related proteins like the host's replication enzymes might further modulate the severity and spread of infection making A4L a potential target for therapeutic intervention to control monkeypox outbreaks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the virion core. Participates in virion assembly.
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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