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AB246902

Anti-Monoacylglycerol Lipase/MGL antibody

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(2 Publications)

Rabbit Polyclonal Monoacylglycerol Lipase/MGL antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MGLL aa 100-300.

View Alternative Names

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Monoacylglycerol Lipase/MGL (green) using ab246902 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)

Paraffin-embedded human colon tissue stained for Monoacylglycerol Lipase/MGL using ab246902 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)
  • WB

Supplier Data

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB246902)

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab246902) at 0.4 µg/mL

All lanes:

U-2197 cell lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MGLL aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q99685

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).
See full target information MGLL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 27: PubMed37144506

2023

MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyong Gu,Mo Chen,Xueyan Li,Dechun Geng,Chen Wang

EBioMedicine 53:102696 PubMed32143183

2020

Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Tang,Hao Xie,Christoph Heier,Jianfei Huang,Qiuling Zheng,Thomas O Eichmann,Gabriele Schoiswohl,Jun Ni,Rudolf Zechner,Songshi Ni,Haiping Hao
View all publications
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Product promise

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