AB24701

Anti-Monoacylglycerol Lipase/MGL antibody

Name: Anti-Monoacylglycerol Lipase/MGL antibody (ab24701) | Abcam
Brand: Abcam Limited
SKU: AB24701
Price: 400 USD
Availability: InStock
Rating: 5 (12 reviews)

(12 Reviews)

(47 Publications)

Rabbit Polyclonal Monoacylglycerol Lipase/MGL antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, African green monkey, Monkey, Human, Cow samples. Cited in 47 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MGLL aa 1-50.

View Alternative Names

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

2 Images

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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

Rat brown fat, 20 ug Dilution : 1/200

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab24701) at 1/200 dilution

All lanes:

Rat brown fat at 20 µg

Predicted band size: 33 kDa

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CiteAb

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

Monoacylglycerol Lipase/MGL western blot using anti-Monoacylglycerol Lipase/MGL antibody ab24701. Publication image and figure legend from Chang, J., Oikawa, S., et al., 2014, Diabetol Metab Syndr, PubMed 24468282.

ab24701 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab24701 please see the product overview.

The amounts of AHSG, CES3, and MGLL in WKY, Lean, and CP rats at 6 and 25 weeks of age. (A) Representative immunoblots of each protein. (B) The amount of each protein quantitated relative to the amount of β-actin and expressed relative to the value of WKY. Data are mean ± SEM of six animals per group. *p < 0.05, versus the corresponding value for WKY. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, African green monkey, Monkey, Human, Cow

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MGLL aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q99685

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.

Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).

See full target information MGLL

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Publications (47)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:3368-3385 PubMed40093888

2025

Sex- and age-specific sensitivities of the endocannabinoid system in Alzheimer's disease revealed by PET imaging with [F]FMPEP- and [F]MAGL-2102.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Pees,Christopher Daniel Morrone,Junchao Tong,Jian Rong,Tuo Shao,Darcy Wear,Steven H Liang,Wai Haung Yu,Neil Vasdev

Journal of neurochemistry 169:e16256 PubMed39556462

2024

Alterations of endocannabinoid signaling and microglia reactivity in the retinas of AD-like mice precede the onset of hippocampal β-amyloid plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Annamaria Tisi,Lucia Scipioni,Giulia Carozza,Lucia Di Re,Giacomo Cimino,Camilla Di Meo,Sakthimala Palaniappan,Francesco Della Valle,Federico Fanti,Giacomo Giacovazzo,Dario Compagnone,Rita Maccarone,Sergio Oddi,Mauro Maccarrone

PloS one 19:e0305868 PubMed38913661

2024

Cannabinoid receptor 1 positive allosteric modulator ZCZ011 shows differential effects on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Hailey Dodson,Shreya Ramineni,Elizabeth Kim,Justin L Poklis,Dai Lu,Bogna M Ignatowska-Jankowska,Aron H Lichtman,Sylvia Fitting

Brain research 1822:148638 PubMed37858856

2023

Effects of acute Δ-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Benjamin L Gorman,Hailey Dodson,Shreya Ramineni,E Diane Wallace,Michelle R Peace,Justin L Poklis,Wei Jiang,Sylvia Fitting

International journal of molecular sciences 24: PubMed37762099

2023

The Effect of Orally Administered Δ9-Tetrahydrocannabinol (THC) and Cannabidiol (CBD) on Obesity Parameters in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Adi Eitan,Ofer Gover,Liron Sulimani,David Meiri,Betty Schwartz

International journal of molecular sciences 24: PubMed37047663

2023

Aβ Chronic Exposure Promotes an Activation State of Microglia through Endocannabinoid Signalling Imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Scipioni,Daniel Tortolani,Francesca Ciaramellano,Federico Fanti,Thais Gazzi,Manuel Sergi,Marc Nazaré,Sergio Oddi,Mauro Maccarrone

International journal of molecular sciences 23: PubMed36499029

2022

Treadmill Exercise Prevents Cognitive Impairments in Adolescent Intermittent Ethanol Rats by Reducing the Excessive Activation of Microglia Cell in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Guo,Min Yan,Li Li,Li Zhao,Yan Li

Animals : an open access journal from MDPI 12: PubMed35327191

2022

Effects of Environmental Heat Load on Endocannabinoid System Components in Adipose Tissue of High Yielding Dairy Cows.

Applications

Unspecified application

Species

Unspecified reactive species

Gitit Kra,Jayasimha Rayalu Daddam,Uzi Moallem,Hadar Kamer,Majdoleen Ahmad,Alina Nemirovski,G Andres Contreras,Joseph Tam,Maya Zachut

Cells 11: PubMed35269478

2022

Inhibitory Neurotransmission Is Sex-Dependently Affected by Tat Expression in Transgenic Mice and Suppressed by the Fatty Acid Amide Hydrolase Enzyme Inhibitor PF3845 via Cannabinoid Type-1 Receptor Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Changqing Xu,Barkha J Yadav-Samudrala,Callie Xu,Bhupendra Nath,Twisha Mistry,Wei Jiang,Micah J Niphakis,Benjamin F Cravatt,Somnath Mukhopadhyay,Aron H Lichtman,Bogna M Ignatowska-Jankowska,Sylvia Fitting

International journal of molecular sciences 22: PubMed34208173

2021

Analysis of Both Lipid Metabolism and Endocannabinoid Signaling Reveals a New Role for Hypothalamic Astrocytes in Maternal Caloric Restriction-Induced Perinatal Programming.

Applications

Unspecified application

Species

Unspecified reactive species

Rubén Tovar,Antonio Vargas,Jesús Aranda,Lourdes Sánchez-Salido,Laura González-González,Julie A Chowen,Fernando Rodríguez de Fonseca,Juan Suárez,Patricia Rivera

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Monoacylglycerol Lipase/MGL antibody

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (47)

Product promise