Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12]

• IHC

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Immunohistochemistry - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Immunohistochemical analysis of paraffin-embedded human adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/5000 (0.098 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human adipose tissue. The section was incubated with ab307162 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

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Immunohistochemistry - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/5000 (0.098 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human liver (PMID : 30034939). The section was incubated with ab307162 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized DU 145 (human prostate carcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/500 dilution (0.976 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in DU 145 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/500 dilution (0.976 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in HT-29 cells line. Low expression : HCT 116 (PMID : 22349814). The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/500 dilution (0.976 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in U-87 MG line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml)  (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized DU145 (human prostate carcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with ab307162 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IP

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Immunoprecipitation - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Monoacylglycerol Lipase/MGL was immunoprecipitated from 0.35 mg DU145 (human prostate carcinoma epithelial cell) whole cell lysate 5 ug with ab307162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : DU145 whole cell lysate 5 ug Lane 2 : ab307162 IP in DU145 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307162 in DU145 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution

Lane 1:

DU145 whole cell lysate at 5 µg

Lane 2:

DU145 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 35 kDa

false

Exposure time: 32s

• WB

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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Buffer/Dilution Concentration : 5% NFDM/TBST Exposure Time : Lane 1 : 10 seconds, Lane 2 : 26 seconds, and Lane 3 : 180 seconds

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution

Lane 1:

U-87 MG whole cell lysate at 20 µg

Lane 2:

PC-3 whole cell lysate at 20 µg

Lane 3:

LNCaP whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Buffer/Dilution Concentration : 5% NFDM/TBST Exposure time : 3 seconds The expression of MGL is down-regulated in response to JZL treatment (PMID : 21802006).

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution

Lane 1:

Untreated DU145 whole cell lysate at 20 µg

Lane 2:

DU145 treated with 1 uM JZL184 for 4 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (AB307162)

Blocking/Dilution Buffer : 5% NFDM/TBST Exposure time : Lane 1 : 26 seconds and Lane 2 : 180 seconds

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution

Lane 1:

Human fat tissue lysate at 20 µg

Lane 2:

Human breast tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 26s