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Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (AB324104), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (AB324104), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (AB324104), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (AB324104), expandable thumbnail
  • Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (AB324104), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Mouse
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Converts monoacylglycerides to free fatty acids and glycerol (PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20554061, PubMed:20729846, PubMed:21454566, PubMed:9341166). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20554061, PubMed:20729846, PubMed:21454566, PubMed:9341166). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).

Alternative names

Monoglyceride lipase, MGL, Monoacylglycerol lipase, MAGL, Mgll

Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29806-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat liver using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoacylglycerol Lipase/MGL with ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in rat liver.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat adipose tissue using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Immunohistochemical analysis of paraffin-embedded Rat adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in rat adipose tissue.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoacylglycerol Lipase/MGL with ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse liver.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse adipose tissue using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Immunohistochemical analysis of paraffin-embedded Mouse adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse adipose tissue.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregation.

    All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104) at 1/1000 dilution

    Lane 1: Rat brown fat tissue lysate at 20 µg

    Lane 2: Rat liver tissue lysate at 20 µg

    Lane 3: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa

    Exposure time: 26s

  • Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregation.

    Exposure time: Lane 1: 6 seconds, Lane 2: 26 seconds

    All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104) at 1/1000 dilution

    Lane 1: Mouse brown fat tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa

  • Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104), expandable thumbnail

    Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104)

    Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti- Monoacylglycerol Lipase/MGL antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21347293; PMID: 23656991), the multiple bands are isoforms.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (ab324104) at 1/1000 dilution

    Lane 1: B16-F10 (mouse skin melanoma cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: B16-F10 transfected with siRNA specifically targeting MGL whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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