Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447007.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447006.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447005.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447004.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239)")
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447016.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (AB324239)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Mouse | Not recommended | Tested | Tested |
| Rat | Not recommended | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Converts monoacylglycerides to free fatty acids and glycerol (PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20554061, PubMed:20729846, PubMed:21454566, PubMed:9341166). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20554061, PubMed:20729846, PubMed:21454566, PubMed:9341166). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).
Alternative names
Monoglyceride lipase, MGL, Monoacylglycerol lipase, MAGL, Mgll
Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29806-56
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324239 is the carrier free version of Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447007.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat liver using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoacylglycerol Lipase/MGL with Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447006.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat adipose tissue using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat adipose tissue.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447005.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoacylglycerol Lipase/MGL with Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447004.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse adipose tissue using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse adipose tissue.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447016.jpg "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregation.
All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104) at 1/1000 dilution
Lane 1: Rat brown fat tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa
Exposure time: 26s
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447015.jpg "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregation.
Exposure time: Lane 1: 6 seconds, Lane 2: 26 seconds
All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104) at 1/1000 dilution
Lane 1: Mouse brown fat tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447014.jpg "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)
Monoacylglycerol Lipase/MGL Western blot staining using rabbit Anti-Monoacylglycerol Lipase/MGL antibody
This data was developed using Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 21347293; PMID: 23656991), the multiple bands are isoforms.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] (Anti- Monoacylglycerol Lipase/MGL antibody [EPR29806-56] ab324104) at 1/1000 dilution
Lane 1: B16-F10 (mouse skin melanoma cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: B16-F10 transfected with siRNA specifically targeting MGL whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447015.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239), expandable thumbnail](https://content.abcam.com/products/images/monoacylglycerol-lipase-mgl-antibody-epr29806-56-bsa-and-azide-free-ab324239--western-blot-img447014.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR29806-56] - BSA and Azide free (ab324239)")
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