AB124796

Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

2 Images

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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (AB124796)

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (ab124796) at 1/1000 dilution

All lanes:

Human adipose tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (AB124796)

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (ab124796) at 1/1000 dilution

All lanes:

Human heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

Carrier free

Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6339

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.

Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).

See full target information MGLL

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 28:12 PubMed35100965

2022

Synergistic effects of ISL1 and KDM6B on non-alcoholic fatty liver disease through the regulation of SNAI1.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhao,Jinjing Ke,Wensheng Pan,Hanghai Pan,Miao Shen

Mediators of inflammation 2021:5754592 PubMed34539243

2021

The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Hu,Hongyan Zai,Wei Jiang,Zhenglin Ou,Yuanbing Yao,Qin Zhu

Scientific reports 9:18944 PubMed31831821

2019

FABP5 coordinates lipid signaling that promotes prostate cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory Carbonetti,Tessa Wilpshaar,Jessie Kroonen,Keith Studholme,Cynthia Converso,Simon d'Oelsnitz,Martin Kaczocha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339]

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (AB124796)

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (AB124796)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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