Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)

Rabbit Recombinant Monoclonal Monoamine Oxidase A/MAO-A antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (AB240031), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Expected
Rat	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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6 products for Alternative Version

Target data

See full target information MAOA

Function

Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed:18391214, PubMed:20493079, PubMed:24169519, PubMed:8316221). Preferentially oxidizes serotonin (PubMed:20493079, PubMed:24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).

Alternative names

Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA

Recommended products

Rabbit Recombinant Monoclonal Monoamine Oxidase A/MAO-A antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR7101
Purification technique: Affinity purification Protein A
Dissociation constant: 5.1 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab240031 is the carrier-free version of Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Monoamine Oxidase A (MAO-A) also known as MAO protein is an enzyme that degrades neurotransmitters through oxidative deamination. It possesses a mass of approximately 59 kDa. This enzyme is found localized in the outer mitochondrial membrane of cells specifically in high concentrations in the brain liver and intestine. MAO-A plays an important role in the breakdown of monoamine neurotransmitters such as serotonin norepinephrine and dopamine which are critical for normal brain function.

Biological function summary

MAO-A acts to maintain proper neurotransmitter levels by catalyzing the oxidative deamination of monoamines. It is not part of a larger protein complex but functions independently to influence monoamine balance. Through its enzymatic activity MAO-A regulates several physiological processes including mood and emotional responses by controlling neurotransmitter availability and preventing their accumulation to neurotoxic levels.

Pathways

MAO-A operates within the catabolic pathways that reduce the bioavailability of monoamines. It pairs with proteins like Catechol-O-methyltransferase (COMT) in the metabolic degradation of catecholamines. This pathway is integral to managing the equilibrium of mood-related neurotransmitters and involves complex interactions with other enzymatic systems including MAO-B which targets different substrates.

Associated diseases and disorders

MAO-A is closely associated with psychiatric conditions such as depression and anxiety. Altered MAO-A activity can dysregulate monoamine levels contributing to these disorders. Additionally research links MAO-A dysregulation to neurodegenerative diseases like Parkinson's disease where imbalances in monoamine levels play a role. This enzyme interacts with proteins involved in early stages of these diseases highlighting its potential as a target for therapeutic interventions using MAO inhibitors.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Immunocytochemistry/ Immunofluorescence - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Monoamine Oxidase A/MAO-A with Purified Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 at 1:100 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Monoamine Oxidase A/MAO-A with Purified Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751)
Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Lanes 1 - 2: Merged signal (red and green). Green - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 observed at 60 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 38 kDa.
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751).
All lanes: Western blot - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 (purified) staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] ab126751).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.